Bioimaging of Glutathione with a Two-Photon Fluorescent Probe and Its Potential Application for Surgery Guide in Laryngeal Cancer
Bioimaging of Glutathione with a Two-Photon Fluorescent Probe and Its Potential Application for Surgery Guide in Laryngeal Cancer
复制标题
双光子荧光探针谷胱甘肽生物成像及其在喉癌手术指南中的潜在应用
DOI：
10.1021/acssensors.9b02118
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发表时间：
2020
期刊：
影响因子：
8.9
通讯作者：
Fabiao Yu
中科院分区：
文献类型：
--
作者：
Yuxia Zou;Mingshun Li;Yanlong Xing;Tingting Duan;Xuejun Zhou;Fabiao Yu
As the most abundant nonprotein biothiol in living cells, glutathione (GSH) prevents cellular components from oxidative damage and maintains the intracellular redox homeostasis. For further exploring whether GSH can be employed as a bioindicator to discriminate tumor lesion at a cellular level, the highly selective detection and accurate quantification of GSH under pathological conditions are critical. Herein, we design a coumarin derivative-based two-photon fluorescent probeCou-Brfor the detection of GSH in living cells, mice models, and clinical specimens. The prepared probe is capable of sensitively and selectively detecting GSH in complex biological systems.Cou-Brdisplays a good linear relationship in response to GSH and a low limit of detection. With the fluorescence signal positively associated with intracellular GSH levels, the probe enables real-time imaging of GSH in various cell lines. Under the condition of CS2stimulation,Cou-Brcan rapidly respond to the fluctuation of intracellular GSH induced by oxidative damage. Furthermore, the in situ and in vivo bioimaging performances ofCou-Brare demonstrated. Typically, relying on the different cellular concentrations of GSH, the probe is successfully employed to identify the human laryngeal cancer lesion with outstanding capabilities of deep tissue imaging and tumor margin recognition. We assume that the abnormal expression level of GSH may be utilized as a potential bioindicator to discriminate tumor tissues from the surrounding disease-free tissues. To conclude, the proposed probeCou-Brmay potentially serve as a powerful chemical tool for the surgical navigation of cancer in clinic.
