Glutathione S-transferase affects permethrin detoxification in the brown dog tick, Rhipicephalus sanguineus
Glutathione S-transferase affects permethrin detoxification in the brown dog tick, Rhipicephalus sanguineus
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DOI：
10.1016/j.ttbdis.2013.11.006
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发表时间：
2014-01-01
影响因子：
3.2
通讯作者：
de la Fuente, Jose
中科院分区：
文献类型：
--
作者：
Duscher, Georg Gerhard;Galindo, Ruth Cecilia;de la Fuente, Jose
控制狗身上的扁虱通常是通过使用含有二氯氰菊酯作为活性成分的驱避剂来完成的。本研究利用基因沉默技术，研究了谷胱甘肽S转移酶基因在二氯氰菊酯解毒过程中的作用。这些研究中使用的棕狗扁虱--血色里皮头虱--对狗有明显的宿主偏好，但也会感染其他哺乳动物。在这项研究中，将GST双链RNA(DsRNA)注射到雌性血吸虫体内，通过RNAi实现基因沉默，然后再暴露于亚致死剂量的氯菊酯。注射60小时后，雌性被允许以绵羊为食。与未经处理的对照相比，接受RNAi的雌蚤被证明对二氯苯菊酯更敏感。基因沉默的效果在最高剂量组(50.3ppm)最显著，在这组中所有的扁虱都死亡了，而在没有接受RNAi的相应对照组中，这个剂量不是致命的。杀螨剂处理后的硬蜱依附行为发生了变化。与紧密聚集在一起并进食的对照组相比，经杀螨剂处理的扁虱以分散的模式附着在一起。与低剂量组和未接触氯菊酯的对照组相比，注射和未注射氯菊酯水平较高的雌性蚊虫的补充所需时间更短，这种更快的附着和摄食可能更有利于病原体的快速传播。然而，试验组之间的充血和蛋重并没有显著差异。这项研究表明，GST基因的沉默增加了硬蜱对二氯氰菊酯的敏感性。总体而言，这些结果有助于我们了解硬蜱的解毒机制，并为制定治疗策略提供新的考虑。(C)2014年爱思唯尔股份有限公司。版权所有。
Control of ticks on dogs is often done by application of repellents that contain permethrin as the active ingredient. In this research, we studied the role of a glutathione S-transferase (GST) gene in detoxification of permethrin by ticks using a gene silencing method RNA interference (RNAi). The brown dog tick, Rhipicephalus sanguineus, used in these studies, has a notable host preference for dogs, but also infests other mammals. In this research, R. sanguineus females were injected with gst double-stranded RNA (dsRNA) to effect gene silencing by RNAi and then exposed to sublethal doses of permethrin. Sixty hours after injection, the females were allowed to feed on sheep. The female ticks subjected to RNAi proved to be more susceptible to permethrin than the untreated controls. The effect of gene silencing was most notable in the highest dose group (50.3 ppm) in which all ticks died, while in the corresponding controls that were not subjected to RNAi this dose was not lethal. The acaricide treatment of the ticks resulted in a change in tick attachment behavior. Acaricide-treated ticks attached in a scattered pattern in contrast to the control ticks that attached and fed tightly clustered together. The time required for repletion for both the injected and non-injected females exposed to the higher permethrin level was shorter than that observed in the lower-dose groups and unexposed controls, and this more rapid attachment and feeding would likely favor more rapid transmission of pathogens. However, engorgement and egg mass weights were not significantly different among the experimental groups. This research demonstrated that the silencing of the gst gene increased the tick's susceptibility to permethrin. Overall, these results have contributed to our understanding of the detoxification mechanism of ticks and provide new considerations for the formulation of treatment strategies. (C) 2014 Elsevier GmbH. All rights reserved.