Heterogeneous expression of the ammonium transporter AgAmt in chemosensory appendages of the malaria vector, Anopheles gambiae.
Heterogeneous expression of the ammonium transporter AgAmt in chemosensory appendages of the malaria vector, Anopheles gambiae.
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疟疾载体冈比亚按蚊化学感应附属物中铵转运蛋白 AgAmt 的异质表达。
DOI：
10.1016/j.ibmb.2020.103360
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发表时间：
2020
影响因子：
3.8
通讯作者：
Zwiebel,LaurenceJ
中科院分区：
文献类型：
--
作者：
Ye,Zi;Liu,Feng;Sun,Huahua;Barker,Mackenzie;Pitts,RJason;Zwiebel,LaurenceJ
氨是源自人类和其他动物散发物的主要利它素之一，在这种情况下，在疟疾媒介蚊子冈比亚按蚊的寻找宿主行为中发挥着重要作用。然而，尽管其在指导宿主寻找方面很重要，但蚊子嗅觉系统中氨检测的机制仍然很大程度上未知。除了持续努力识别和表征构成氨敏感性的分子受体外，先前的研究还揭示了铵转运蛋白（Amt）在调节果蝇触角和行为反应中的重要作用。冈比亚。在前者中，DmAmtin触角感觉器定位于氨感觉神经元周围的辅助细胞，导致了这样的假设：其作用是清除感觉器淋巴中多余的铵离子。在后者中，RT-PCR和异源表达已被用来检查An的表达和功能特征。冈比亚铵转运蛋白，AgAmt。我们现在采用先进的转基因工具来全面检查幼虫和成年雌性 An 外周化学感应附属物的 AgAmt 空间定位。冈比亚。在幼虫触角中，AgAmt 出现在神经元和辅助细胞中。与D相反。在黑腹果蝇的成虫触角中，在非神经元辅助细胞以及氨反应性基感器和腔感器的感觉神经元中都观察到了 AgAmt 衍生的信号。在上颌须、唇瓣和跗骨中，AgAmt 似乎仅限于感觉神经元。我们还描述了成体触角腔室感器和上颌须头状钉对氨的反应，揭示了感受器AgAmt表达与氨敏感性之间的相关性。总而言之，这些数据表明 AgAmt 可能在成虫和幼虫化学感应装置中发挥异质作用，并且作为蚊子控制中的超受体靶标具有潜在的广泛用途。
Ammonia is one of the principal kairomones originating from human and other animal emanations and in that context, plays an essential role in the host-seeking behaviors of the malaria vector mosquito Anopheles gambiae. Nevertheless, despite its importance in directing host-seeking, the mechanisms underlying ammonia detection in the mosquito olfactory system remains largely unknown. In addition to ongoing efforts to identify and characterize the molecular receptors that underlie ammonia sensitivity, previous studies have revealed a prominent role for ammonium transporters (Amt) in modulating antennal and behavioral responses in Drosophila melanogaster andAn. gambiae. In the former, localization ofDmAmtin antennal sensilla to auxiliary cells surrounding the ammonia sensory neurons led to the hypothesis that its role was to clear excess ammonium ions in the sensillar lymph. In the latter, RT-PCR and heterologous expression have been used to examine the expression and functional characteristics of theAn. gambiaeammonium transporter,AgAmt. We now employ advanced transgenic tools to comprehensively examineAgAmtspatial localization across the peripheral chemosensory appendages in larvae and adult femaleAn. gambiae. In the larval antennae,AgAmtappears localized in both neuronal and auxiliary cells. In contrast toD. melanogaster, in the adult antennae,AgAmt-derived signals are observed in both non-neuronal auxiliary cells and in sensory neurons in ammonia-responsive basiconic and coeloconic sensilla. In the maxillary palps, labella, and tarsi,AgAmtappears restricted to sensory neurons. We have also characterized the responses to ammonia of adult antennal coeloconic sensilla and maxillary palp capitate pegs revealing a correlation between sensillarAgAmtexpression and ammonia sensitivity. Taken together, these data suggest thatAgAmtmay play heterogeneous roles in the adult and larval chemosensory apparatus and potentially broad utility as a supra-receptor target in mosquito control.