Identification of a PTC-containing DlRan transcript and its differential expression during somatic embryogenesis in Dimocarpus longan
Identification of a PTC-containing DlRan transcript and its differential expression during somatic embryogenesis in Dimocarpus longan
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龙眼体细胞胚胎发生过程中含 PTC 的 DlRan 转录物的鉴定及其差异表达
DOI：
10.1016/j.gene.2013.07.091
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发表时间：
2013-10-15
期刊：
影响因子：
3.5
通讯作者：
Lai, Zhong-Xiong
中科院分区：
文献类型：
--
作者：
Fang, Zhi-Zhen;Lai, Cheng-Chun;Lai, Zhong-Xiong
RAN （ras相关核蛋白）在酵母、动物和植物的多种细胞过程中起着至关重要的作用。本文报道了从龙眼胚性培养中鉴定出的含有过早终止密码子（ptc）的DlRan基因及其选择性剪接转录本。多重比对和剪接模式分析表明，含有FTC的DlRan-1转录本是选择性剪接的结果。当翻译抑制剂环己亚胺处理胚性愈伤组织时，含有DlRan ptc的转录本的积累显著增加，表明DlRan-1可能是NMD的靶点。通过对DlRan转录本表达谱的分析发现，在龙眼体细胞胚发生过程中，不同剪接的DlRan转录本在胚性愈伤组织、球形胚和子叶形胚的发育过程中分别存在差异表达水平，且与相应野生型转录本的胚胎发育过程一致。本研究为推测含有ftc的转录本的选择性剪接可能具有功能性，并可能揭示龙眼体细胞胚胎发育过程中DlRan的表达调控提供了证据。(C) 2013 Elsevier B.V.版权所有
RAN (Ras-related nuclear protein) plays crucial roles in multiple cellular processes in yeast, animals and plants. Here we present a DlRan gene and its alternative splicing transcripts containing premature terminator codons (PTCs), identified from embryogenic cultures in longan. Multiple alignment and splicing pattern analyses indicated that DlRan-1 transcript harboring FTC was the consequence of alternative splicing. The accumulation of DlRan PTC-containing transcripts increased significantly when the embryogenic calli were treated with the translation inhibitor, cycloheximide, indicating that DlRan-1 may be targeted by NMD. The analysis of expression profiles of DlRan transcripts revealed that differential expression levels of the alternative spliced DlRan transcripts occurred during the development of embryogenic callus, globular-shaped embryos, and cotyledon-shaped embryos, respectively, in the longan somatic embryogenesis, and were in consistent with the embryo development in corresponding wild-type transcripts. The present work offers evidence to speculate that the alternatively spliced FTC-containing transcripts can be functional and may shed light on expression regulation of DlRan during development of the longan somatic embryos. (C) 2013 Elsevier B.V. All rights reserved.