Oxidative stress and antioxidant status in patients with autoimmune liver diseases
Oxidative stress and antioxidant status in patients with autoimmune liver diseases
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DOI：
10.1179/1351000214y.0000000101
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发表时间：
2015-01-01
期刊：
影响因子：
3.8
通讯作者：
Moulas, Anargyros N.
中科院分区：
文献类型：
--
作者：
Kaffe, Eleanna T.;Rigopoulou, Eirini I.;Moulas, Anargyros N.
目的：评估自身免疫性肝病不同阶段的氧化应激和抗氧化成分，并评估其对疾病进展的可能影响。方法：我们测定了氧化损伤的几个标志物（异前列烷、醛类、蛋白质羰基、3-硝基酪氨酸和髓过氧化物酶）和抗氧化成分（谷胱甘肽、谷胱甘肽过氧化物酶、谷胱甘肽还原酶、超氧化物歧化酶和过氧化氢酶），对49例自身免疫性胆汁淤积性肝病（AC）患者和36例自身免疫性肝炎（AIH）患者及年龄、性别相匹配的健康对照者进行了尿微量白蛋白测定。与对照组相比，AC和AIH患者的所有脂质和蛋白质氧化损伤产物水平均升高，全血谷胱甘肽水平显著降低。与AC患者相比，AIH患者的醛类和谷胱甘肽过氧化物酶活性水平显著较高，蛋白质羰基水平显著较低。AC和AIH患者中，随着从轻度纤维化到重度纤维化和肝硬化的进展，蛋白质羰基和异前列烷水平逐渐升高，谷胱甘肽水平逐渐降低。此外，AC和AIH患者有显着更高的蛋白质carbonyls相比，non-apartics.Discussion：我们提供了新的研究结果，支持在AC和AIH的肝损伤的进展中的氧化剂/抗氧化剂失衡的主要贡献。
Objective: To estimate oxidative stress and antioxidant components during different stages of autoimmune liver diseases and assess their possible implication on disease progression.Methods: We determined several markers of oxidative injury (isoprostane, aldehydes, protein carbonyls, 3-nitrotyrosine, and myeloperoxidase) and antioxidant components (glutathione, glutathione peroxidase, glutathione reductase, superoxide dismutase, and catalase) in whole blood, serum, and urine in 49 patients with autoimmune cholestatic liver diseases (AC) and 36 patients with autoimmune hepatitis (AIH) and healthy subjects matched for sex and age.Results: Both AC and AIH patients had increased levels of all lipid and protein oxidative injury products and significantly decreased whole blood glutathione levels compared to controls. AIH patients had significantly higher levels of aldehydes and glutathione peroxidase activity and significantly lower protein carbonyl levels compared to AC patients. Protein carbonyl and isoprostane levels increased and glutathione levels decreased gradually with progression from mild fibrosis to severe fibrosis and cirrhosis in both AC and AIH patients. In addition, both cirrhotic AC and AIH patients had significantly higher protein carbonyls compared to non-cirrhotics.Discussion: We provide novel findings in support of a major contribution of oxidant/antioxidant imbalance in the progression of liver injury in AC and AIH.