Two orphan receptors binding to a common site are involved in the regulation of the oxytocin gene in the bovine ovary.
Two orphan receptors binding to a common site are involved in the regulation of the oxytocin gene in the bovine ovary.
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两个孤儿受体结合到一个共同位点，参与牛卵巢催产素基因的调节。
DOI：
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发表时间：
1994
影响因子：
11.1
通讯作者：
N. Walther
中科院分区：
文献类型：
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作者：
U. Wehrenberg;Richard Ivell;Martina Jansen;S. Goedecke;N. Walther
肽激素催产素在下丘脑中仅在少数大细胞神经元内高度表达。然而，它也以发情周期依赖的方式在牛黄体中以高水平在大量细胞中表达。通过使用核提取物从这个组织在体外结合研究，两个蛋白质复合物已被证明结合到一个共同的网站在牛催产素启动子。这些蛋白质之一已被确定为鸡卵清蛋白上游启动子转录因子（COUP-TF）的牛同源物。第二种蛋白质在此被表征为组织特异性转录因子类固醇生成因子1（SF-1）的牛同源物。在黄体发育过程中这两种因子的相对表达与黄体催产素基因表达水平相关，当催产素基因启动子被激活时，SF-1是与该启动子结合的因子。使用小鼠睾丸细胞系TM 4的共转染实验表明，SF-1可以刺激转染的催产素基因的表达，这表明SF-1可能参与催产素基因在体内的上调，可能通过将刺激信号转导到RNA聚合酶。
The peptide hormone oxytocin is highly expressed in the hypothalamus within only a small number of magnocellular neurons. However, it is also expressed in a much larger number of cells in the bovine corpus luteum at high levels in an estrous cycle-dependent manner. By using nuclear extracts from this tissue for in vitro binding studies, two protein complexes have been shown to bind to a common site in the bovine oxytocin promoter. One of these proteins has been identified as the bovine homologue of the chicken ovalbumin upstream promoter transcription factor (COUP-TF). The second protein is here characterized as the bovine homologue of a tissue-specific transcription factor, steroidogenic factor 1 (SF-1). The relative expression of these two factors during luteal development correlates with the level of luteal oxytocin gene expression, with SF-1 being the factor binding to the promoter of the oxytocin gene when this promoter is activated. Cotransfection experiments using the murine testicular cell line TM4 show that SF-1 can stimulate the expression of a transfected oxytocin gene, suggesting that SF-1 may be involved in upregulation of the oxytocin gene in vivo, possibly by transducing a stimulatory signal to the RNA polymerase.