The β-subunit composition of nicotinic acetylcholine receptors in the neurons of the guinea pig inferior mesenteric ganglion
The β-subunit composition of nicotinic acetylcholine receptors in the neurons of the guinea pig inferior mesenteric ganglion
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DOI：
10.1016/j.neulet.2004.04.071
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发表时间：
2004-07-22
影响因子：
2.5
通讯作者：
Skok, VI
中科院分区：
文献类型：
--
作者：
Koval, OM;Voitenko, LP;Skok, VI
使用针对大鼠神经元烟碱乙酰胆碱受体 β2 和 β4 亚基合成 (183-192) 片段的抗体，通过免疫细胞化学染色和阻断电刺激节前神经（肠系间干）诱导的兴奋性突触后电位，研究豚鼠肠系膜下神经节烟碱受体 β 亚基组成。 beta4 特异性抗体对 59.8 +/- 7.5% 的神经元进行染色，并抑制了 25.5 +/- 1.8% 所研究的所有 (n = 10) 神经元的突触反应。 β2特异性抗体不会对神经节神经元染色，也不影响突触传递。考虑到先前获得的关于该神经节中发现的α亚基的数据，得出的结论是，肠系膜下神经节的神经元含有参与肠系膜间道突触传递的α3（α5）β4亚型烟碱受体。 (C) 2004 Elsevier Ireland Ltd. 保留所有权利。
The antibodies against synthetic (183-192) fragments of beta2- and beta4-subunits of rat neuronal nicotinic acetylcholine receptor were used to study a beta-subunit composition of nicotinic receptors in the inferior mesenteric ganglion of the guinea pig by both immunocytochemical staining and blocking of excitatory postsynaptic potentials induced by electric stimulation of the pre-ganglionic nerve (intermesenteric trunk). The beta4-specific antibody stained 59.8 +/- 7.5% of neurons and inhibited the synaptic responses in all (n = 10) neurons studied by 25.5 +/- 1.8%. The beta2-specific antibody did not stain ganglionic neurons and did not affect the synaptic transmission. Taking into account the previously obtained data on the alpha-subunits found in this ganglion, it is concluded that the neurons of inferior mesenteric ganglion contain nicotinic receptors of alpha3(alpha5)beta4 subtypes involved in synaptic transmission through the intermesenteric tract. (C) 2004 Elsevier Ireland Ltd. All rights reserved.