Validity of a single antibody-based lateral flow immunoassay depending on graphene oxide for highly sensitive determination of E. coli O157:H7 in minced beef and river water
Validity of a single antibody-based lateral flow immunoassay depending on graphene oxide for highly sensitive determination of E. coli O157:H7 in minced beef and river water
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DOI：
10.1016/j.foodchem.2019.124965
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发表时间：
2019-11-01
期刊：
影响因子：
8.8
通讯作者：
Mekoci, Arben
中科院分区：
文献类型：
--
作者：
Abdelazeem Hassan, Abdelrahim Hussein;Francisco Bergua, Jose;Mekoci, Arben
考虑到E.大肠杆菌O 157：H7在食品和水中的存在，一个负担得起的和高灵敏度的检测方法是至关重要的。在此，我们报告了基于单抗体的荧光侧流免疫测定法（FLFIA）的首次使用，该免疫测定法依赖于氧化石墨烯和量子点之间的非辐射能量转移来测定大肠杆菌。大肠杆菌O 157：H7。FLFIA显示出比常规侧向流（LF）高千倍的高灵敏度。在接种的碎牛肉和河水样本中，检测限分别为178和133 CFU g（-1）或mL（-1）。此外，在其他可能的干扰菌存在下，它表现出高选择性。单抗体方法将测定成本降低到比常规LF低60%。此外，结果可以通过便携式LF阅读器或智能手机读取。这些优点使FLFIA成为食品和水中病原体检测的一种有前途的技术。
Considering the health risks of E. coli O157:H7 presence in food and water, an affordable and highly sensitive detection method is crucial. Herein, we report the first use of a single antibody-based fluorescent lateral flow immunoassay (FLFIA) depending on non-radiative energy transfer between graphene oxide and quantum dots for determination of E. coli O157: H7 in beef and river water. FLFIA showed a high sensitivity rate thousand-fold better than the conventional lateral flow (LF). In inoculated minced beef and river water samples, the limits of detection were 178 and 133 CFU g(-1) or mL(-1), respectively. Besides, it presented a high selectivity in the presence of other possible interfering bacteria. The single antibody approach reduced the assay cost to 60% less than the conventional LF. Alongside, the results could be read by portable LF readers or smartphones. These advantages offer FLFIA as a promising technology for pathogen detection in food and water.