Upregulated expression and activation of membrane-associated proteases in esophageal squamous cell carcinoma
Upregulated expression and activation of membrane-associated proteases in esophageal squamous cell carcinoma
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DOI：
10.3892/or.2014.3162
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发表时间：
2014-06-01
期刊：
影响因子：
4.2
通讯作者：
Sikorski, Aleksander F.
中科院分区：
文献类型：
--
作者：
Augoff, Katarzyna;Hryniewicz-Jankowska, Anita;Sikorski, Aleksander F.
为了更好地了解膜相关蛋白水解系统在食管癌发生发展中的作用，我们研究了两种丝氨酸蛋白酶、成纤维细胞活化蛋白-α（FAP-α）和二肽基肽酶IV（DPPIV）以及三种金属蛋白酶，基质金属蛋白酶（MMP）-2、MMP-9和MT1-MMP在24个原发性食管鳞状细胞癌（ESCC）组织和配对的非癌组织。通过逆转录 PCR、蛋白质印迹和酶谱分析，我们发现丝氨酸蛋白酶和所有三种金属蛋白酶在食管鳞癌中都发生了高度改变。发现 FAP-α 和 DPPIV 的表达与两种明胶酶的活性呈正相关。这可能表明这些蛋白水解系统彼此紧密相连，共同参与促进癌细胞侵袭和转移的 ECM 降解过程。
To better understand the role of membrane-associated proteolytic systems in the development of esophageal cancer, we studied the expression of two serine proteases, fibroblast activation protein-alpha (FAP-alpha) and dipeptidyl peptidase IV (DPPIV) and three metalloproteinases, matrix metalloproteinase (MMP)-2, MMP-9 and MT1-MMP in 24 primary esophageal squamous cell carcinoma (ESCC) tissues and paired non-cancer tissues. Using reverse-transcription PCR, western blotting and zymography, we showed that both serine proteases and all three metalloproteinases were highly altered in ESCC. A positive correlation between the expression of FAP-alpha and DPPIV and the activity of both gelatinases was found. This may indicate that these proteolytic systems are tightly linked to each other and collectively are involved in the process of ECM degradation that facilitates cancer cell invasion and metastasis.