Egg hatching activity of trihydroxylated eicosanoids in the barnacle Balanus balanoides.
Egg hatching activity of trihydroxylated eicosanoids in the barnacle Balanus balanoides.
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藤壶 Balanus balanoides 中三羟基类二十烷酸的卵孵化活性。
DOI：
10.1016/0304-4165(93)90113-m
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
East,J
中科院分区：
文献类型：
--
作者：
Hill,EM;Holland,DL;East,J
以前的工作表明，二十烷类孵化因子可以从各种藤壶物种的组织中分离出来。为了进一步研究藤壶孵化因子的性质，从藤壶幼虫匀浆中分离得到二十烷类化合物，用反相高效液相色谱法和三羟基脂肪酸组分进行纯化，并对其孵化活性进行了生物活性测定和GC-MS分析。鉴定为8，11，12-三羟基-5，9，14-二十碳三烯酸、8，11，12-三羟基-59，14，17-二十碳四烯酸、10，11，12-trihydroxy-5，8，14，17-eicosatetraenoic酸和10，13，14-trihydroxy-4，7，11，16，19-docosapentaenoic酸。这些研究表明，藤壶具有强大的n-9脂氧合酶，可以代谢花生四烯酸、二十碳五烯酸或二十二碳六烯酸。所有活性部位共有的主要二十烷类化合物是8，11，12-三羟基二十碳三烯酸或8，11-12-三羟基二十碳四烯酸的异构体，这表明8，11，12三醇的特定构型是藤壶孵化因子。
Previous work has shown that eicosanoid hatching factors can be isolated from tissues of a variety of barnacle species. To further investigate the nature of barnacle hatching factor an eicosanoid fraction isolated from homogenates of the barnacleBalanus balanoideswas purified on RP-HPLC and trihydroxy fatty acid fractions, were bioassayed for hatching activity and analysed by GC-MS. The identified eicosanoids were 8,11,12-trihydroxy-5,9,14-eicosatrienoic acid, 8,11,12-trihydroxy-59,14,17-eicosatetraenoic acid, 10,11,12-trihydroxy-5,8,14,17-eicosatetraenoic acid and 10,13,14-trihydroxy-4,7,11,16,19-docosapentaenoic acid. These studies suggest that barnacles possess a potent n-9 lipoxygenase which can metabolise either arachidonic acid, eicosapentaenoic acid or docosahexaenoic acid. The predominant eicosanoids common to all active fractions were either 8,11,12-trihydroxyeicosatrienoic acid or an isomer of 8,11–12-trihydroxyeicosatetraenoic acid, which suggests that a particular configuration of an 8,11, 12 triol is barnacle hatching factor.