PLATELET DENSE GRANULE RELEASE REACTION MONITORED BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY FLUOROMETRIC-DETERMINATION OF ENDOGENOUS SEROTONIN
PLATELET DENSE GRANULE RELEASE REACTION MONITORED BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY FLUOROMETRIC-DETERMINATION OF ENDOGENOUS SEROTONIN
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DOI：
10.1016/s0003-2697(05)80011-9
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发表时间：
1992-10-01
影响因子：
2.9
通讯作者：
COHEN, DJ
中科院分区：
文献类型：
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作者：
ANDERSON, GM;HALL, LM;COHEN, DJ
通过测量释放后过滤收集的血小板的5-羟色胺含量来监测血小板致密颗粒释放反应。灵敏的HPLC-荧光分析允许使用小体积（25-50 μl）血浆和全血评估释放。由于过滤作用的快速停止，可在短至1秒的时间内检查释放。凝血酶刺激（1 U/ml）释放的半衰期为2-3 s，对于稀释的全血和血浆样品，在60 s释放期内观察到的凝血酶刺激释放的EC 50值为0.01-0.03 U/ml。
The platelet dense granule release reaction was monitored by measuring the serotonin content of platelets collected by filtration following release. Sensitive HPLC-fluorometric analysis permitted release to be assessed using small (25–50 μl) volumes of plasma and whole blood. Release could be examined for periods as short as 1 s due to the rapid stoppage effected by filtration. The half-life of thrombin-stimulated (1 U/ml) release was 2–3 s, the EC50values observed for thrombinstimulated release over a 60-s release period were 0.01–0.03 U/ml for diluted whole blood and plasma samples.