Metabolic responses in gills of Manila clam Ruditapes philippinarum exposed to copper using NMR-based metabolomics.
Metabolic responses in gills of Manila clam Ruditapes philippinarum exposed to copper using NMR-based metabolomics.
复制标题
DOI：
10.1016/j.marenvres.2011.04.002
复制
发表时间：
2011-07
影响因子：
3.3
通讯作者：
Linbao Zhang;Xiaoli Liu;L. You;Di Zhou;Huifeng Wu;Lianzhen Li;Jianmin Zhao;Jianghua Feng
中科院分区：
文献类型：
--
作者：
Linbao Zhang;Xiaoli Liu;L. You;Di Zhou;Huifeng Wu;Lianzhen Li;Jianmin Zhao;Jianghua Feng
铜是渤海海域一种重要的高生态风险重金属污染物。本研究采用核磁共振代谢组学方法，研究了生物指标马尼拉蛤（Ruditapes philippinarum）对环境相关铜暴露的代谢反应。铜暴露引起的显著代谢变化与渗透物、克雷布斯循环中间体和氨基酸有关，如暴露96 h时，蛤鳃中己甘氨酸、支链氨基酸含量增加，琥珀酸、丙氨酸和二甲胺含量减少。综上所述，Cu在96 h的实验期间可能导致蛤的渗透调节和能量代谢紊乱。这些结果表明，基于核磁共振的代谢组学可用于发现代谢生物标志物，并可用于阐明海洋重金属污染物的毒理学机制。
Copper is an important heavy metal contaminant with high ecological risk in the Bohai Sea. In this study, the metabolic responses in the bioindicator, Manila clam (Ruditapes philippinarum), to the environmentally relevant copper exposures were characterized using NMR-based metabolomics. The significant metabolic changes corresponding to copper exposures were related to osmolytes, intermediates of the Krebs cycle and amino acids, such as the increase in homarine, branched chain amino acids and decrease in succinate, alanine and dimethylamine in the copper-exposed clam gills during 96 h exposure period. Overall, Cu may lead to the disturbances in osmotic regulation and energy metabolism in clams during 96 h experimental period. These results demonstrate that NMR-based metabolomics is applicable for the discovery of metabolic biomarkers which could be used to elucidate the toxicological mechanisms of marine heavy metal contaminants.