Molecular cloning of a cDNA for the glutamate transporter of the nematode Caenorhabditis elegans.
Molecular cloning of a cDNA for the glutamate transporter of the nematode Caenorhabditis elegans.
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线虫秀丽隐杆线虫谷氨酸转运蛋白 cDNA 的分子克隆。
DOI：
10.1006/bbrc.1996.1676
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发表时间：
1996
影响因子：
3.1
通讯作者：
T. Nakajima
中科院分区：
文献类型：
--
作者：
T. Kawano;K. Takuwa;T. Nakajima
我们克隆了线虫线虫的谷氨酸转运蛋白的cDNA。核苷酸序列测定和北方杂交结果表明，谷氨酸转运蛋白基因在C. elegans中，随后的反式剪接分别产生谷氨酸转运蛋白和ATP合成酶c亚基的两种不同的单顺反子mRNA。这些单顺反子mRNA的产量差异很大，表明C. elegans在转录后阶段受到调控。C.线虫与哺乳动物的序列相似性约为50-60%。这是对无脊椎动物谷氨酸转运蛋白的首次描述。
We cloned a cDNA for the glutamate transporter of the nematode Caenorhabditis elegans. The nucleotide sequence and Northern blotting indicated that the glutamate transporter gene is transcribed as a polycistronic mRNA in C. elegans and that subsequent trans-splicing yields two distinct monocistronic mRNAs for the glutamate transporter and the ATP synthase c subunit, respectively. The yields of these monocistronic mRNAs were quite different, suggesting that glutamate transport in C. elegans is regulated in the post-transcriptional phase. The glutamate transporter of C. elegans shows about 50-60% sequence similarity with those of mammals. This is the first description of invertebrate glutamate transporters.