Some properties of the interaction of follicle stimulating hormone with bovine granulosa cells and its inhibition by follicular fluid.
Some properties of the interaction of follicle stimulating hormone with bovine granulosa cells and its inhibition by follicular fluid.
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促卵泡激素与牛颗粒细胞相互作用的一些特性及其受卵泡液抑制的作用。
DOI：
10.1095/biolreprod19.2.235
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发表时间：
1978
影响因子：
3.6
通讯作者：
L. Reichert
中科院分区：
文献类型：
--
作者：
N. Darga;L. Reichert
我们研究了放射性标记的人促卵泡激素（1251-hFSH）的结合牛颗粒细胞收集卵泡在不同阶段的成熟判断的大小。特异性结合是时间和温度依赖性的，在37 ° C和pH 7.5孵育2小时后达到最大值。特异性结合相对于1251-hFSH和受体浓度是饱和的，并且可以被未标记的纯化人FSH（25 ng = 50%抑制）和牛FSH（35 ng = 50%抑制）抑制，但不被大量（5000 ng）牛LH、TSH、GH、催乳素或人ACTH抑制。251-hFSH与大卵泡（>6 mm）颗粒细胞的特异性结合至少与中卵泡（3-6 mm）和小卵泡（<3 mm）颗粒细胞的特异性结合一样大。所有大小的牛卵泡液均以剂量相关方式显著抑制1251-hFSH与颗粒细胞的结合。来自大卵泡的FSH结合抑制（FSH-Bl）/ml流体的量比来自小卵泡的约2倍，并且由于增加的流体体积，所含的FSH-Bl活性水平比小卵泡高约100倍。FSH-B1活性通过透析显著降低，通过具有8000道尔顿的分子量保留的膜，并且可以在透析液中检测到。可透析卵泡液FSH-B1的化学性质尚不清楚，尽管它似乎不被活性炭吸附或不溶于乙醚。
We have studied the binding of radiolabeled human follicle stimulating hormone (1251-hFSH) to bovine granulosa cells collected from follicles at varying stages of maturation as judged by size. Specific binding was time and temperature dependent, reaching its maximum after 2 h of incubation at 37#{176}Cand pH 7.5. Specific binding was saturable with respect to 1251-hFSH and receptor concentrations and could be inhibited by unlabeled purified human FSH (25 ng = 50% inhibition) and bovine FSH (35 ng = 50% inhibition), but not by large (5000 ng) amounts of bovine LH, TSH, GH, prolactin or human ACTH. Specific binding of 2 51-hFSH to granulosa cells from large follicles (>6 mm) was at least as great as to granulosa cells from medium (3-6 mm) and small (<3 mm) follicles. Fluid from bovine follicles of all sizes significantly inhibited binding of 1251-hFSH to granulosa cells in a dose related manner. The amount of FSH binding inhibition (FSH-Bl)/ml of fluid from large follicles was approximately 2-fold greater than that from small follicles and contained approximately 100-fold higher levels of FSH-Bl activity than did small follicles by virtue of increased fluid volume. FSH-Bl activity was markedly reduced by dialysis, passing a membrane having a molecular weight retention of 8000 daltons and could be detected in the dialysate. The chemical nature of the dialyzable follicular fluid FSH-Bl is not known, although it does not seem to be adsorbed by charcoal or soluble in diethyl ether.