Home dust microbiota is disordered in homes of low-income asthmatic children.
Home dust microbiota is disordered in homes of low-income asthmatic children.
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DOI：
10.3109/02770903.2015.1028076
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Rosenwasser L
中科院分区：
文献类型：
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作者：
Ciaccio CE;Barnes C;Kennedy K;Chan M;Portnoy J;Rosenwasser L
人们一再发现，接触微生物会影响哮喘等特应性疾病的发展。已经开发出了能够全面描述微生物群落特征的创新技术。本研究的目的是利用基于16S rRNA的基因芯片系统发育分析来描述哮喘儿童的家庭微生物区系。在这项横断面研究中，从家居粉尘中提取DNA，并扩增细菌16S rRNA基因。细菌产品被杂交到PhyloChip阵列，并使用基因阵列扫描仪进行扫描。用Adonis检验来确定整个微生物组的显著差异。用韦尔奇t检验确定显著丰度差异和属水平丰富度差异。19个家庭被纳入分析(14个哮喘家庭，5个非哮喘家庭)。在至少一个样本中发现了1741个可操作分类单位(OTU)。哮喘组和非哮喘组的细菌属丰富度无显著差异(p=0.09)。两组之间的微生物谱有显著差异(p=0.025)。在哮喘患者家庭中，前12个丰度差异显著的OTU全部增加，属于五大门类之一(p=0.001~p=7.2x10−6)。近一半的显著丰度差异属于蓝藻或变形细菌门。这些结果表明，家庭粉尘具有一种特有的微生物群，这种微生物群在哮喘患者的家中受到干扰，导致蓝藻和变形杆菌的数量特别丰富。还需要进一步的研究，利用高通量技术进一步阐明家庭微生物暴露如何影响人类健康和疾病。
Exposure to microorganisms has repeatedly been found to influence development of atopic diseases, such as asthma. Innovative techniques have been developed that can comprehensively characterize microbial communities. The objective of this study was to characterize the home microbiota of asthmatic children utilizing 16S rRNA based phylogenetic analysis by microarray. In this cross-sectional study DNA was extracted from home dust and bacterial 16S rRNA genes amplified. Bacterial products were hybridized to the PhyloChip Array and scanned using a GeneArray scanner. The Adonis test was used to determine significant differences in the whole microbiome. Welch’s t-test was used to determine significant abundance differences and genus-level richness differences. Nineteen homes were included in the analysis (14 asthma, 5 no asthma). 1741 operational taxonomic units (OTUs) were found in at least one sample. Bacterial genus richness did not differ in the homes of asthmatics and non-asthmatics (p=0.09). The microbial profile was significantly different between the two groups (p=0.025). All of the top 12 OTUs with significant abundance differences were increased in homes of asthmatics and belonged to one of the five phyla (p=0.001 to p=7.2 × 10−6). Nearly half of significant abundance differences belonged to the phylum Cyanobacteria or Proteobacteria. These results suggest that home dust has a characteristic microbiota which is disturbed in the homes of asthmatics, resulting in a particular abundance of Cyanobacteria and Proteobacteria. Further investigations are needed which utilize high throughput technology to further clarify how home microbial exposures influence human health and disease.