Radiosensitive melanoma cell line with mutation of the gene for ataxia telangiectasia.
Radiosensitive melanoma cell line with mutation of the gene for ataxia telangiectasia.
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DOI：
10.1038/bjc.1998.2
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发表时间：
1998
影响因子：
8.8
通讯作者：
Lavin, M
中科院分区：
文献类型：
--
作者：
Ramsay, J;Birrell, G;Baumann, K;Bodero, A;Parsons, P;Lavin, M
检测了人黑色素瘤细胞系MM96L、A2058和HT144对电离辐射和UVB辐射的敏感性。与MM96L和A2058细胞相比，HT144细胞对电离辐射和UVB辐射的敏感性显著增加。对这两种药物的敏感性与细胞凋亡的易感性有关。利用蛋白质截断分析，在HT144细胞中发现了共济失调毛细血管扩张症(ATM)基因的突变。随后对异常区域进行测序，证实这是一种纯合子突变。另外两个细胞系的蛋白质截断分析显示没有异常。结果提示，A-T基因的体细胞突变在确定肿瘤放射敏感性方面可能具有重要意义。
The human melanoma cell lines MM96L, A2058 and HT144 were examined for sensitivity to ionizing radiation and UVB radiation. HT144 demonstrated a significant increase in sensitivity to ionizing and UVB radiation compared with the MM96L and A2058 cells. Sensitivity to both agents was associated with susceptibility to apoptosis. Using a protein truncation assay, a mutation for the gene for ataxia telangiectasia (ATM) was identified in HT144 cells. This was confirmed to be a homozygous mutation by subsequent sequencing of the abnormal region. Protein truncation assay of the other two cell lines showed no abnormality. The results suggest that somatic mutation of the A-T gene may be important in determining tumour radiosensitivity.