Structure and expression of the rice class-I type histone deacetylase genes OsHDAC1-3:: OsHDAC1 overexpression in transgenic plants leads to increased growth rate and altered architecture
Structure and expression of the rice class-I type histone deacetylase genes OsHDAC1-3:: OsHDAC1 overexpression in transgenic plants leads to increased growth rate and altered architecture
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DOI：
10.1046/j.1365-313x.2003.01650.x
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发表时间：
2003-02-01
期刊：
影响因子：
7.2
通讯作者：
Kim, JK
中科院分区：
文献类型：
--
作者：
Jang, IC;Pahk, YM;Kim, JK
组蛋白去乙酰化酶（HDAC）调节染色质结构和转录。HDAC已经被研究为真核生物转录中的负调控因子。我们分离了水稻OsHDAC 1 -3中与RPD 3家族相关的I类组蛋白去乙酰化酶基因。OsHDAC 1基因编码约57 kDa的蛋白质，其与OsHDAC 2、OsHDAC 3、玉米RPD 3和人HDAC 1蛋白质分别具有73.5%、72.7%、79.9%和57.1%的氨基酸序列同一性。基因组结构和Southern杂交分析表明，OsHDAC 1 -3分别含有7个、6个和7个外显子，构成水稻基因组中的I类家族。OsHDAC 1在叶、根和愈伤组织细胞中表达水平相似，而OsHDAC 2和3在根和愈伤组织细胞中表达，但在叶中不表达，表现出明显的组织特异性。为了探索组蛋白脱乙酰酶在转基因植物中的作用，我们将OsHDAC 1 cDNA片段插入到ABA诱导型启动子Ai调控下的表达载体Ai：：OsHDAC 1中，并转化水稻。在Ai：：OsHDAC 1愈伤组织细胞中，mRNA、蛋白质和HDAC活性水平显著增加。转基因细胞中四乙酰化H4的量大大减少，并且在用阿司他汀A处理后消除了这种减少。这些结果表明，OsHDAC 1在转基因细胞中的过表达产生酶活性的HDAC复合物，并诱导体内组蛋白乙酰化的变化。过表达导致一系列新的表型，包括增加的生长速率和改变的植物结构，表明OsHDAC 1在基因表达的全基因组编程中起作用。
Histone deacetylases (HDACs) modulate chromatin structure and transcription. HDACs have been studied as negative regulators in eukaryotic transcription. We isolated the rice OsHDAC1-3 genes for class I-type histone deacetylases, which are related to the RPD3 family. The OsHDAC1 gene encoded a protein of approximately 57 kDa that shared 73.5, 72.7, 79.9, and 57.1% amino acid sequence identity with the OsHDAC2, OsHDAC3, maize RPD3, and human HDAC1 proteins, respectively. Genomic structures and Southern blot analyses revealed that OsHDAC1-3 contained seven, six, and seven exons, respectively, and constituted a class I-type family in the rice genome. OsHDAC1 was expressed at similar levels in the leaves, roots, and callus cells, whereas OsHDAC2 and 3 were expressed in the roots and callus cells, but not in the leaves, exhibiting distinct tissue specificity. To explore the role of histone deacetylases in transgenic plants, we inserted the OsHDAC1 cDNA fragment into the expression vector Ai::OsHDAC1 under the control of the ABA-inducible promoter Ai, and transformed the construct into rice. Levels of mRNA, protein, and HDAC activity were significantly increased in Ai::OsHDAC1 callus cells. The amount of tetra-acetylated H4 in the transgenic cells was greatly reduced, and the reduction was abolished upon treatment with trichostatin A. These results demonstrate that OsHDAC1 overexpression in transgenic cells both yields enzymatically active HDAC complexes and induces changes in histone acetylation in vivo. The overexpression leads to a range of novel phenotypes, involving increased growth rate and altered plant architecture, suggesting that OsHDAC1 functions in the genome-wide programming of gene expression.