Laminin peptide-conjugated chitosan membrane: Application for keratinocyte delivery in wounded skin
Laminin peptide-conjugated chitosan membrane: Application for keratinocyte delivery in wounded skin
复制标题
DOI：
10.1002/jbm.a.30804
复制
发表时间：
2006-12-01
影响因子：
4.9
通讯作者：
Kadoya, Yuichi
中科院分区：
文献类型：
--
作者：
Ikemoto, Shigehiro;Mochizuki, Mayumi;Kadoya, Yuichi
组织工程需要将细胞运送到需要修复的器官部位并使其存活。以前，我们表明，活性层粘连蛋白肽（AG 73：RKR-LQVQLSIRT）结合的壳聚糖膜促进细胞粘附和体外扩散。在这里，我们将人角质形成细胞接种到AG 73-壳聚糖膜上，发现近80%的细胞在2小时内附着在膜上。将携带角质形成细胞的膜倒置并置于裸鼠背部暴露的肌肉筋膜上。3天后，角质形成细胞已从膜上迁移，并在筋膜上建立了分层的表皮样结构。细胞通过跨膜蛋白聚糖多配体识别AG 73，该识别系统以前未在组织工程应用中进行过测试。我们认为AG 73-壳聚糖膜可用作治疗制剂，并且可用作细胞递送系统，例如将角质形成细胞递送至伤口床。(c)2006 Wiley Periodicals，Inc.
Tissue engineering requires the delivery and survival of cells to organ sites needing repair. Previously, we showed that an active laminin peptide (AG73: RKR-LQVQLSIRT)-conjugated chitosan membrane promoted cell adhesion and spreading in vitro. Here, we seeded human keratinocytes onto AG73-chitosan membranes and found that nearly 80% of the cells were attached to the membranes within 2 h. The membranes carrying the keratinocytes were inverted and placed onto exposed muscle fascia on the backs of nude mice. After 3 days, the keratinocytes had migrated from the membrane and established a stratified epidermis-like structure on the fascia. Cells recognize the AG73 through transmembrane proteoglycan syndecans, which recognition system has not previously been tested in tissue engineering applications. We suggest that the AG73-chitosan membrane is useful as a therapeutic formulation and is applicable as a cell delivery system such as delivering keratinocytes to a wound bed. (c) 2006 Wiley Periodicals, Inc.