Study on the adsorption of DNA on the layered double hydroxides (LDHs).
Study on the adsorption of DNA on the layered double hydroxides (LDHs).
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DOI：
10.1016/j.saa.2013.10.099
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发表时间：
2014-03
期刊：
影响因子：
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通讯作者：
Bin Li;Ping-xiao Wu;Bo Ruan;Paiyu Liu;Nengwu Zhu
中科院分区：
文献类型：
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作者：
Bin Li;Ping-xiao Wu;Bo Ruan;Paiyu Liu;Nengwu Zhu
采用化学共沉淀法制备了四种层状双金属氢氧化物（LDHs），并将其用作DNA吸附剂。采用X射线粉末衍射（XRD）、傅里叶变换红外光谱（FTIR）和标准电子模块（SEM）等多种表征手段对LDHs进行了表征。通过考察初始浓度、溶液pH值等因素对LDHs吸附DNA的影响，研究LDHs对DNA的吸附性能。结果表明，Mg/Al = 3的LDHs对DNA的吸附能力较强，且不受pH值的影响。结果表明，LDHs对DNA具有良好的吸附性能，在2 h内吸附完全。吸附平衡数据分别用Langmuir和Freundlich模型拟合，表明Langmuir模型代表单分子层吸附，与吸附线性方程有较好的相关性。圆二色谱（CD）、紫外-可见光谱（UV-vis）和琼脂糖凝胶电泳结果表明，吸附过程中DNA结构没有受到损伤。
Four kinds of layered double hydroxides (LDHs) were prepared by chemical coprecipitation method and used as DNA adsorbents. Multiple characterization tools such as power X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy and Standard electronic modules (SEM) were employed to characterize the LDHs. By examining the effect of initial concentration, solution pH, adsorption experiments were carried out to investigate the adsorption capacities of LDHs for DNA. The results revealed that the LDHs with Mg/Al = 3 had higher ability on adsorbing the DNA and were not affected by pH values. The LDHs exhibited excellent adsorption properties and completely adsorbed DNA within 2 h. The adsorption equilibrium data were fitted to the Langmuir and Freundlich models, showing that the Langmuir model which represented monolayer adsorption had better correlation with the adsorption linear equation. In addition, Circular dichroism (CD) spectrum, UV–vis spectorscopy and agarose gel electrophoresis revealed the integrity of DNA structure, suggesting that there had no damage on the DNA structure during the adsorption process.