ISOLATION OF BOVINE CORONAVIRUS FROM FECES AND NASAL SWABS OF CALVES WITH DIARRHEA
ISOLATION OF BOVINE CORONAVIRUS FROM FECES AND NASAL SWABS OF CALVES WITH DIARRHEA
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DOI：
10.1292/jvms.53.433
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发表时间：
1991-06-01
影响因子：
1.2
通讯作者：
SHIMIZU, M
中科院分区：
文献类型：
--
作者：
TSUNEMITSU, H;YONEMICHI, H;SHIMIZU, M
采集了180头腹泻犊牛和36头临床正常同居者的粪便和鼻腔样本，并用来自人直肠腺癌的HRT-18细胞培养进行了病毒检测。从腹泻犊牛的5份粪便和56份鼻腔标本中分离到一株细胞病变病毒。所有从腹泻粪便中分离出病毒的牛犊从鼻拭子中分离出病毒呈阳性。该病毒也是从10头临床正常的牛犊的鼻拭子中分离出来的，这些牛犊与腹泻的牛犊同居。由于它们在形态上与冠状病毒相似，凝集小鼠红细胞，在血清学上与内布拉斯加州小牛腹泻冠状病毒相同，因此新分离株被鉴定为牛冠状病毒。用直接免疫荧光法显示鼻腔上皮细胞中有病毒抗原，与HRT-18细胞培养中的病毒分离结果非常一致。这是日本首次从腹泻新生牛群中分离到牛冠状病毒。从鼻拭子中经常分离到该病毒的证据对了解牛冠状病毒感染的发病机制具有重要意义。
Fecal and nasal samples were collected from 180 calves with diarrhea and 36 clinically normal co-habitants, and tested for virus using HRT-18 cell cultures derived from human rectal adenocarcinoma. A cytopathic virus was isolated from 5 fecal and 56 nasal samples obtained from diarrheic calves. All calves in which the virus was isolated from diarrheic feces were positive for virus isolation from nasal swabs. The virus was also isolated from the nasal swabs of 10 clinically normal calves that were co-habitants with diarrheic calves. Because they were morphologically similar to coronavirus, agglutinated mouse erythrocytes and serologically identical with the Nebraska calf diarrhea coronavirus, new isolates were identified as bovine coronavirus. The demonstration of viral antigens in nasal epithelial cells by a direct immunofluorescence was in close agreement with the virus isolation in HRT-18 cell cultures. This is the first report on the isolation of bovine coronavirus from newborn calves with diarrhea in Japan. The evidence that the virus was frequently isolated from nasal swabs is of great interest for understanding the pathogenesis of bovine coronavirus infection.