DEMONSTRATION OF POLY-N-ACETYL LACTOSAMINE RESIDUES IN AMEBOID AND RAMIFIED MICROGLIAL CELLS IN RAT-BRAIN BY TOMATO LECTIN-BINDING
DEMONSTRATION OF POLY-N-ACETYL LACTOSAMINE RESIDUES IN AMEBOID AND RAMIFIED MICROGLIAL CELLS IN RAT-BRAIN BY TOMATO LECTIN-BINDING
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DOI：
10.1177/42.8.8027523
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发表时间：
1994-08-01
影响因子：
3.2
通讯作者：
CASTELLANO, B
中科院分区：
文献类型：
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作者：
ACARIN, L;VELA, JM;CASTELLANO, B
本研究旨在证明多n -乙酰基乳胺残基在出生后和成年大鼠大脑中的定位，通过它们与从番茄中获得的凝集素的特异性结合来可视化。在低温恒温器、石蜡和振动体切片上进行凝集素组织化学，并进行光镜检查。选定的振动体切片用于电子显微镜。结果表明，番茄凝集素组织化学与大鼠脑血管和神经胶质细胞有关。由于番茄凝集素阳性的胶质细胞不表现出GFAP免疫反应性，并且表现出与核苷二磷酸酶（NDPase）阳性细胞相同的形态特征和总体分布，因此它们被鉴定为小胶质细胞。在电镜下，变形虫细胞和分枝小胶质细胞均表现出胞浆内和质膜凝集素反应性。在出生后的大脑中，变形虫小胶质细胞与番茄凝集素的结合比成人大脑中的分枝小胶质细胞更强。本文讨论了从变形虫到分枝小胶质细胞中聚n -乙酰乳胺减少的推测意义以及这种糖渣的可能作用。
This study was designed to demonstrate the localization of poly-N-acetyl lactosamine residues in postnatal and adult rat brain, visualized by their specific binding to a lectin obtained from Lycopersicon esculentum (tomato). Lectin histochemistry was carried out on cryostat, paraffin, and vibratome sections and was examined by light microscopy. Selected vibratome sections were professed for electron microscopy. Our results showed that tomato lectin histochemistry was found in relation to blood vessels and glial cells in both postnatal and adult rat brain. Since tomato lectin-positive glial cells did not show GFAP immunoreactivity and displayed the same morphological features and overall distribution as nucleoside diphosphatase (NDPase)-positive cells, they were consequently identified as microglial cells. At the electron microscopic level, both ameboid and ramified microglial cells displayed intracytoplasmic and plasma membrane lectin reactivity. In postnatal brain, ameboid microglial cells always showed stronger binding of tomato lectin compared with ramified microglial cells in the adult brain. The putative significance of this decrease in poly-N-acetyl lactosamine from ameboid to ramified microglial cells and the possible role(s) of this sugar residue are discussed.