Biodegradation of phenanthrene by Pseudomonas sp BZ-3, isolated from crude oil contaminated soil
Biodegradation of phenanthrene by Pseudomonas sp BZ-3, isolated from crude oil contaminated soil
复制标题
从原油污染土壤中分离的假单胞菌 BZ-3 对菲的生物降解
DOI：
10.1016/j.ibiod.2014.07.011
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发表时间：
2014-10-01
影响因子：
4.8
通讯作者：
Wang, Chuanyuan
中科院分区：
文献类型：
--
作者：
Lin, Meng;Hu, Xiaoke;Wang, Chuanyuan
从滨州原油污染土壤中分离到一株菲降解菌BZ-3。根据16S rRNA基因序列鉴定该分离物为假单胞菌BZ-3。通过实验考察了pH、盐度和PHE浓度对PHE降解效率的影响。采用气相色谱-质谱和高效液相色谱-质谱分析检测PHE的降解效率和降解代谢物。菌株BZ-3在初始浓度为50 mg/L、盐度为20 g/L条件下，7 d内可降解75%的PHE。采用一级降解速率模型估计PHE降解动力学，计算速率系数为0.108 d（-1）。根据鉴定的降解代谢物，菌株BZ-3可以通过水杨酸代谢途径降解PHE。在由PHE和萘（NA）、蒽（ANTH）、芘（PYR）等多环芳烃组成的混合体系中，菌株BZ-3表现出高效的降解能力。进一步研究表明，菌株BZ-3也能以NA、ANTH、PYR、二甲苯、1-羟基-2-环烷和己烷作为唯一的碳源和能量源，但不能在含硝基苯的培养基上生长。(C) 2014 Elsevier Ltd.版权所有。
A phenanthrene (PHE) degrading bacterium strain BZ-3 was isolated from the crude oil contaminated soil in Binzhou, China. The isolate was identified as Pseudomonas sp. BZ-3 on the basis of 16S rRNA gene sequence. Various experiments were conducted to investigate the effect of pH, salinity and PHE concentration on the degradation efficiency of PHE. The degradation efficiency and degradation metabolites of PHE were detected by using GC-MS and HPLC-MS analyses. The strain BZ-3 could degrade 75% of PHE at an initial concentration of 50 mg/L under 20 g/L salinity in 7 days. PHE degradation kinetics was estimated in a first-order degradation rate model and the rate coefficient was calculated as 0.108 d(-1). On the basis of the identified degradation metabolites, the strain BZ-3 could degrade PHE in the salicylate metabolic pathway. In a mixture system consisting of PHE and other PAHs including naphthalene (NA), anthracene (ANTH), and pyrene (PYR), the strain BZ-3 showed an efficiently degradation capability. Further study showed that the strain BZ-3 could also use NA, ANTH, PYR, xylene, 1-hydroxy-2-naphthoic acid, and hexane as the sole carbon and energy source, but did not grow on nitrobenzene-containing medium. (C) 2014 Elsevier Ltd. All rights reserved.