Flow cytometry: A new method to investigate the properties of water-in-oil-in-water emulsions
Flow cytometry: A new method to investigate the properties of water-in-oil-in-water emulsions
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DOI：
10.1021/la035402
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发表时间：
2004-03-16
期刊：
影响因子：
3.9
通讯作者：
Magdassi, S
中科院分区：
文献类型：
--
作者：
Hai, M;Bernath, K;Magdassi, S
我们提出了一种新的简便方法，通过流式细胞术评估含有荧光标记的 w/o/w 乳液。流式细胞术可以同时测量用荧光标记“标记”或“空白”的w/o/w乳液液滴，无需复杂的样品制备。采用该新方法考察了w/o/w乳液的制备产率和荧光标记FITC-BSA的释放率。随着内相内 FITC-BSA 浓度的增加，w/o/w 乳液中 FITC-BSA 的释放分数（24 小时后）降低，就像 w/o/w 乳液中作为标记的 NaCl 释放分数一样。流式细胞术结果表明，w/o/w 乳液中的产率和释放行为与更复杂的方法报告的结果一致。
We present a new and facile method to evaluate w/o/w emulsions containing fluorescent markers by flow cytometry. Flow cytometry allows simultaneous measurement of w/o/w emulsion droplets "marked" with a fluorescent marker or "blank" without the need for complicated sample preparation. The yield of preparation of the w/o/w emulsion and the release rate of the fluorescent marker FITC-BSA were investigated by this new method. The release fraction (after 24 h) of FITC-BSA from the w/o/w emulsion decreased with increasing concentration of FITC-BSA inside the internal phase, just like the release fraction of NaCl as marker from the w/o/w emulsion. Flow cytometry results show that the yield and release behavior in w/o/w emulsions are in agreement with results reported by more complicated methods.