2P or not 2P: The Question of Seizure Initiation.
2P or not 2P: The Question of Seizure Initiation.
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DOI：
10.1177/1535759720941023
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发表时间：
2020-07-20
影响因子：
3.6
通讯作者：
Goldberg EM
中科院分区：
文献类型：
--
作者：
Somarowthu A;Goldberg EM
张文，张文军. Ann Neurol。2020;87（1）：97-115。doi：10.1002/ana.25628研究癫痫发作间期棘波和癫痫发作差异启动的网络动力学机制。我们进行了结合体内2光子钙成像从不同的目标神经元亚群和细胞外电生理记录在4-氨基吡啶诱导的新皮层尖峰和癫痫发作。棘波和癫痫发作都与兴奋性第2/3层锥体神经元（PN）和较小程度的第4层神经元以及抑制性小清蛋白表达中间神经元（IN）的强烈同步激活相关。与此形成鲜明对比的是，在癫痫发作过程中，第5层PN和生长抑素表达的IN逐渐异步地被招募到发作活动中。在2/3层内，尖峰发作和癫痫发作之间的主要差异在于兴奋性PN的相对募集动力学相比，小清蛋白和生长抑素表达抑制性INs. While尖峰表现出平衡的PN和小清蛋白表达IN的募集，在癫痫发作期间IN反应减少，同步性低于2/3层PN。在新皮层的第4层或第5层中没有观察到类似的不平衡。我们开发的基于机器学习的算法能够仅基于放电开始时2/3层PN的激活动力学来区分尖峰和癫痫发作。释义：在癫痫发作期间，神经元亚群之间的募集动力学明显不同，第2/3层PN、第4层神经元和表达小白蛋白的IN快速同步募集，第5层PN和表达生长抑素的IN逐渐异步募集。由于局部PN和抑制性IN之间的动态失配，并且随后通过逐渐且异步地招募该层中的PN来传播到第五层。
Layer- and Cell-Specific Recruitment Dynamics During Epileptic Seizures In Vivo Aeed F, Shnitzer T, Talmon R, Schiller Y. Ann Neurol. 2020;87(1):97-115. doi:10.1002/ana.25628 To investigate the network dynamics mechanisms underlying differential initiation of epileptic interictal spikes and seizures. We performed combined in vivo 2-photon calcium imaging from different targeted neuronal subpopulations and extracellular electrophysiological recordings during 4-aminopyridine-induced neocortical spikes and seizures. Both spikes and seizures were associated with intense synchronized activation of excitatory layer 2/3 pyramidal neurons (PNs) and to a lesser degree layer 4 neurons, as well as inhibitory parvalbumin-expressing interneurons (INs). In sharp contrast, layer 5 PNs and somatostatin-expressing INs were gradually and asynchronously recruited into the ictal activity during the course of seizures. Within layer 2/3, the main difference between onset of spikes and seizures lay in the relative recruitment dynamics of excitatory PNs compared to parvalbumin- and somatostatin-expressing inhibitory INs. Whereas spikes exhibited balanced recruitment of PNs and parvalbumin-expressing INs, during seizures IN responses were reduced and less synchronized than in layer 2/3 PNs. Similar imbalance was not observed in layers 4 or 5 of the neocortex. Machine learning-based algorithms we developed were able to distinguish spikes from seizures based solely on activation dynamics of layer 2/3 PNs at discharge onset. Interpretation: During onset of seizures, the recruitment dynamics markedly differed between neuronal subpopulations, with rapid synchronous recruitment of layer 2/3 PNs, layer 4 neurons, and parvalbumin-expressing INs and gradual asynchronous recruitment of layer 5 PNs and somatostatin-expressing INs. Seizures initiated in layer 2/3 due to a dynamic mismatch between local PNs and inhibitory INs, and only later spread to layer 5 by gradually and asynchronously recruiting PNs in this layer.
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影响因子：
5.3
作者：
Tran, Conny H.;Vaiana, Michael;Goldberg, Ethan M.
通讯作者：
Goldberg, Ethan M.
影响因子：
5.3
作者：
Wenzel, Michael;Hamm, Jordan P.;Yuste, Rafael
通讯作者：
Yuste, Rafael
影响因子：
8.8
作者：
Wenzel M;Hamm JP;Peterka DS;Yuste R
通讯作者：
Yuste R
影响因子：
64.8
作者：
Jun JJ;Steinmetz NA;Siegle JH;Denman DJ;Bauza M;Barbarits B;Lee AK;Anastassiou CA;Andrei A;Aydın Ç;Barbic M;Blanche TJ;Bonin V;Couto J;Dutta B;Gratiy SL;Gutnisky DA;Häusser M;Karsh B;Ledochowitsch P;Lopez CM;Mitelut C;Musa S;Okun M;Pachitariu M;Putzeys J;Rich PD;Rossant C;Sun WL;Svoboda K;Carandini M;Harris KD;Koch C;O'Keefe J;Harris TD
通讯作者：
Harris TD
影响因子：
11.2
作者：
Aeed, Fadi;Shnitzer, Tal;Schiller, Yitzhak
通讯作者：
Schiller, Yitzhak