Mosaic structure of the penA gene in the oropharynx of men who have sex with men negative for gonorrhoea
Mosaic structure of the penA gene in the oropharynx of men who have sex with men negative for gonorrhoea
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DOI：
10.1177/0956462419889265
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发表时间：
2020-01-30
影响因子：
1.4
通讯作者：
Re, Maria Carla
中科院分区：
文献类型：
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作者：
Marangoni, Antonella;Marziali, Giacomo;Re, Maria Carla
口咽是淋病奈瑟菌产生多重耐药性的关键部位。嵌合佩纳等位基因与头孢菌素敏感性降低相关，通过与部分佩纳基因的DNA重组而出现，特别是那些来自咽部奈瑟菌属种的基因。在这里，我们调查了佩纳基因嵌合体结构在与咽部淋病检测阴性的男性发生性关系的男性口咽部的患病率。2016年1月至2018年6月，在意大利的性传播感染诊所招募了351名淋病阴性男性。咽拭子进行实时聚合酶链反应（PCR）检测嵌合型佩纳基因。在阳性的情况下，对PCR产物进行测序，并针对奈瑟菌菌株的几个序列进行搜索。总的来说，31例患者（8.8%）被发现存在镶嵌佩纳基因阳性。阳性与既往咽部淋病病例（相对危险度[RR]：3.56，95%置信区间1.44-8.80）和近期β-内酰胺类药物暴露（RR：4.29，95%置信区间2.20-8.38）显著相关。所有penA阳性样本均显示与镶嵌阳性奈瑟菌菌株的高度相关性（90-99%）。我们的数据强调，口咽部的嗜酸奈瑟菌可能是赋予奈瑟菌耐药性的遗传物质的重要储存库。淋病
The oropharynx represents a crucial site for the emergence of multi-drug resistance in Neisseria gonorrhoeae. The mosaic penA alleles, associated with decreased susceptibility to cephalosporins, have emerged by DNA recombination with partial penA genes, particularly those from commensal pharyngeal Neisseria species. Here, we investigated the prevalence of the mosaic structure of the penA gene in the oropharynx of men who have sex with men testing negative for pharyngeal gonorrhoea. From January 2016 to June 2018, 351 gonorrhoea-negative men who have sex with men attending a sexually transmitted infection clinic in Italy were enrolled. Pharyngeal swabs underwent a real-time polymerase chain reaction (PCR) for the detection of the mosaic penA gene. In case of positivity, PCR products were sequenced and searched against several sequences of Neisseria strains. Overall, 31 patients (8.8%) were found positive for the presence of the mosaic penA gene. The positivity was significantly associated with previous cases of pharyngeal gonorrhoea (relative risk [RR]: 3.56, 95% confidence interval 1.44-8.80) and with recent exposure to beta-lactams (RR: 4.29, 95% confidence interval 2.20-8.38). All penA-positive samples showed a high relatedness (90-99%) with mosaic-positive Neisseria strains. Our data underline that commensal Neisseria species of the oropharynx may be a significant reservoir for genetic material conferring antimicrobial resistance in N. gonorrhoeae.