Simultaneous time-varying systemic appearance of oral and hepatic glucose in adults monitored with stable isotopes
Simultaneous time-varying systemic appearance of oral and hepatic glucose in adults monitored with stable isotopes
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DOI：
10.1152/ajpendo.1998.275.4.e717
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发表时间：
1998-10-01
影响因子：
5.1
通讯作者：
Greenwood, RH
中科院分区：
文献类型：
--
作者：
Livesey, G;Wilson, PDG;Greenwood, RH
口服葡萄糖负荷和肝脏（R-aO，R-aH）动脉化血浆中葡萄糖的出现率（和程度）可以使用放射性同位素在人体中估计，但估计值因实验室而异。我们研究了稳定同位素的使用，并在健康人中进行了22次预充静脉输注D-[6，6（2）H（2）]葡萄糖，口服负荷包括D-[C-13（6）]葡萄糖。有效葡萄糖池体积（Vs）的下限为230 ml/kg体重（参见通常假定为130 ml/kg）。与Steele的葡萄糖动力学一室模型相比，每70 kg体重50 g口服葡萄糖负荷的全身表现为摄入量的96 +/- 51%，与理论值相似，为95%。Mari的二室模型给出了100 +/-3%。两种模型在每个时间点给出几乎相同的R-aO和R-aH，并且当吸收完成时累积R-aO达到平台。少于3%的C-13被再循环为[C-13（3）]葡萄糖，表明在本研究中再循环误差几乎可以忽略不计。实验室之间的变化的原因进行了鉴定。我们的结论是，稳定同位素提供了一个可靠和安全的替代放射性同位素在这些研究中。
The rates (and extent) of appearance of glucose in arterialized plasma from an oral glucose load and from liver (R-aO, R-aH) can be estimated in humans using radioisotopes, but estimates vary among laboratories. We investigated the use of stable isotopes and undertook 22 primed intravenous infusions of D-[6,6(2)H(2)]glucose with an oral load including D-[C-13(6)]glucose in healthy humans. The effective glucose pool volume (Vs) had a lower limit of 230 ml/kg body weight (cf. 130 ml/kg commonly assumed). This Vs in Steele's one-compartment model of glucose kinetics gave a systemic appearance from a 50-g oral glucose load per 70 kg body weight of 96 +/- 51% of that ingested, which compared with a theoretical value of similar to 95%. Mari's two-compartment model gave 100 +/- 3%. The two models gave practically identical R-aO and R-aH at each point in time and a plateau in the cumulative R-aO when absorption was complete. Less than 3% of C-13 was recycled to [C-13(3)]glucose, suggesting that recycling errors were practically negligible in this study. Causes of variation among laboratories are identified. We conclude that stable isotopes provide a reliable and safe alternative to radioactive isotopes in these studies.