Cloning of rat eotaxin: ozone inhalation increases mRNA and protein expression in lungs of brown Norway rats
Cloning of rat eotaxin: ozone inhalation increases mRNA and protein expression in lungs of brown Norway rats
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DOI：
10.1152/ajplung.1998.274.1.l171
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发表时间：
1998-01-01
影响因子：
4.9
通讯作者：
Hasegawa, S
中科院分区：
文献类型：
--
作者：
Ishii, Y;Shirato, M;Hasegawa, S
C-C趋化因子eotaxin被认为在豚鼠、小鼠和人类中选择性募集嗜酸性粒细胞至炎症部位中是重要的。我们分离了大鼠嗜酸性粒细胞趋化因子基因，以确定是否有类似的分子可能在大鼠急性炎症过程中嗜酸性粒细胞的肺浸润中发挥作用。大鼠嗜酸细胞活化趋化因子的cDNA编码一个97个氨基酸的蛋白质，其中含有一个74个氨基酸的成熟嗜酸细胞活化趋化因子蛋白，与小鼠嗜酸细胞活化趋化因子具有97.3%的同一性。该基因编码的重组蛋白在微趋化室中显示出对嗜酸性粒细胞的特异性趋化活性。Eotaxin mRNA的表达在臭氧暴露后立即增加了1.6倍，20 h后增加了4倍。相同时间点的可灌洗嗜酸性粒细胞数量分别是对照嗜酸性粒细胞的3倍和15倍。免疫细胞化学显示肺泡巨噬细胞和支气管上皮细胞呈嗜酸性粒细胞趋化因子阳性。这些结果表明，嗜酸性粒细胞活化趋化因子可能参与招募嗜酸性粒细胞进入空气空间在某些炎症条件下在大鼠。
The C-C chemokine eotaxin is thought to be important in the selective recruitment of eosinophils to the site of inflammation in guinea pigs, mice, and humans. We isolated the rat eotaxin gene to determine whether a similar molecule might play a role in the pulmonary infiltration of eosinophils during acute inflammation in the rat. The cDNA for rat eotaxin encoded a 97-amino acid protein containing a 74-amino acid mature eotaxin protein with 97.3% identity to mouse eotaxin. The recombinant protein encoded by this gene displayed specific chemotactic activity for eosinophils when analyzed with a microchemotactic chamber. The expression of eotaxin mRNA increased similar to 1.6-fold immediately after exposure to ozone and was 4-fold higher after 20 h. The number of lavageable eosinophils at the same time points were 3- and 15-fold greater, respectively, than control eosinophils. Immunocytochemistry revealed that alveolar macrophages and bronchial epithelial cells were positive for eotaxin. These results suggest that eotaxin may be involved in the recruitment of eosinophils into the air spaces during certain inflammatory conditions in rats.