Tannic Acid and Iron Alum with Safranin and Orange G in Studies of the Shoot Apex
Tannic Acid and Iron Alum with Safranin and Orange G in Studies of the Shoot Apex
复制标题
单宁酸和铁明矾与番红和橙 G 在芽尖研究中的应用
DOI：
10.3109/10520294309105799
复制
发表时间：
1943
影响因子：
1.6
通讯作者：
B. C. Shabman
中科院分区：
文献类型：
--
作者：
B. C. Shabman
本文给出了原生质体用番红和橙子G染色后，用鞣酸和铁矾对幼植物组织细胞壁染色的时间表。将切片在2%ZnCl2水溶液中放置1分钟，然后在番红O的1/25，000水溶液中染色。将它们在由橙子G（2 g）组成的浴中放置5分钟，单宁酸（5 g.）。水（高达100 cc）和HCl（4滴）。然后在5%鞣酸水溶液中浸泡5分钟，在1%铁明矾溶液中浸泡2分钟。在每个阶段之间用自来水进行简短的冲洗;每次更换时，载玻片都要升降十几次，以确保新溶液快速到达材料。该方法起源于茎尖，但它也适用于更成熟的组织和成人材料。它的优点是，即使在维管分化开始时，也可以非常容易地检测到原韧皮部。
A schedule is given for staining the cell walls of young plant tissues in tannic acid and iron alum after the protoplasts have been stained in safranin and orange G. Sections are placed for one minute in 2% aqueous ZnCl2, and are then stained in a 1/25,000 aqueous solution of safranin O. From this they are placed for five minutes in a bath consisting of orange G (2 g.), tannic acid (5 g.). water (up to 100 cc.) and HC1 (4 drops). This is followed by five minutes in 5% aqueous tannic acid and two minutes in a 1% solution of iron alum. A brief rinse in tap water is given between each stage; the slides are raised and lowered about a dozen times at each change to ensure that the new solution reaches the material quickly. The method was originated for shoot apices but it also works excellently on more mature tissues and on adult material. It has the advantage of allowing extremely easy detection of protophloem in the strands even at the very onset of vascular differentiation.