The role of sFRP4, a secreted frizzled-related protein, in ovulation
The role of sFRP4, a secreted frizzled-related protein, in ovulation
复制标题
DOI：
10.1023/a:1024181203729
复制
发表时间：
2003-08-01
期刊：
影响因子：
7.2
通讯作者：
Dharmarajan, AM
中科院分区：
文献类型：
--
作者：
Drake, JM;Friis, RR;Dharmarajan, AM
排卵是一个复杂的多因素事件，涉及卵泡和卵巢表面特定区域通过细胞凋亡而退化。卵巢中已鉴定出许多与细胞凋亡相关的基因。分泌性卷曲相关蛋白 4 (sFRP4) 是一种似乎拮抗细胞存活分子途径的蛋白质。已知 sFRP4 基因表达随着卵巢黄体细胞凋亡而上调。在这项研究中，通过激素诱导未成熟 Wistar 大鼠排卵，并收集其卵巢用于细胞凋亡和 sFRP4 分析。与对照组相比，TUNEL 染色发现处理后的大鼠卵巢膜层中有更多数量的死亡细胞。 3'端标记结果显示，与其他时间点和对照相比，治疗后 12 小时细胞凋亡显着增加 (p < 0.01)。原位杂交显示，与对照组相比，治疗组大鼠毛囊鞘层中 sFRP4 mRNA 表达量明显增加。定量 RT-PCR 显示处理组和对照组织之间 sFRP4 表达水平没有显着差异，但在处理组中观察到明显的增加趋势。这项研究证明了 sFRP4 与大鼠排卵细胞凋亡之间的关联。
Ovulation is a complex, multi-factorial event that involves the degeneration of a specific area of the follicular and ovarian surface via apoptosis. Many apoptosis related genes have been identified in the ovary. Secreted Frizzled Related Protein 4 (sFRP4) is a protein that appears to antagonize a molecular pathway for cell survival. sFRP4 gene expression is known to be upregulated with apoptosis in the ovarian corpus luteum. In this study, ovulation was hormonally induced in immature Wistar rats and their ovaries collected for analysis of apoptosis and sFRP4. TUNEL staining identified a greater amount of dying cells in the thecal layer of treated rat ovaries compared to controls. The results of 3'-end labelling revealed a significant increase (p < 0.01) in apoptosis at 12 hours following treatment compared to other time points and control. In situ hybridization exhibited a visible increase in amounts of sFRP4 mRNA expression in the thecal layers of follicles from treated rats compared to controls. Quantitative RT-PCR revealed no significant difference in sFRP4 expression levels between treated and control tissues although a clear trend towards an increase was observed in the treated group. This study demonstrates an association between sFRP4 and apoptosis in rat ovulation.