Molecular recognition-mediated fabrication of protein nanostructures by dip-pen lithography
Molecular recognition-mediated fabrication of protein nanostructures by dip-pen lithography
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DOI：
10.1021/n10257364
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发表时间：
2002-11-01
期刊：
影响因子：
10.8
通讯作者：
Zauscher, S
中科院分区：
文献类型：
--
作者：
Hyun, J;Ahn, SJ;Zauscher, S
我们描述了分子生物学介导的，逐步制造图案化的蛋白质纳米结构的特征尺寸为200 nm的顺序。首先，16-巯基十六烷酸（MHA）的自组装单层（SAM）通过蘸笔纳米光刻法（DPN）图案化到金上，并且未图案化的区域用抗蛋白质的低聚乙二醇封端的烷基乙醇SAM钝化。接下来，胺封端的生物素衍生物与化学活化的MHA SAM纳米图案共价缀合。然后将表面与链霉抗生物素蛋白一起孵育以形成链霉抗生物素蛋白纳米结构，这是由生物素和链霉抗生物素蛋白之间的分子识别介导的。最后，通过分子印迹法将生物素化的蛋白质从溶液中固定化，制备了蛋白质纳米粒子。我们的制造方法是普遍适用的，因为生物素标记的分子无处不在。
We describe the molecular recognition-mediated, stepwise fabrication of patterned protein nanostructures with feature sizes on the order of 200 nm. First, a self-assembled monolayer (SAM) of 16-mercaptohexadecanoic acid (MHA) is patterned onto gold by dip-pen nanolithography (DPN), and the unpatterned regions are passivated with a protein-resistant oligoethylene glycol-terminated alkanethiol SAM. Next, an amine-terminated biotin derivative is covalently conjugated with the chemically activated MHA SAM nanopattern. The surface is then incubated with streptavidin to form streptavidin nanostructures, mediated by molecular recognition between biotin and streptavidin. Finally, protein nanopatterns are fabricated by molecular recognition-mediated immobilization of biotinylated protein from solution. Our fabrication methodology is generically applicable because of the ubiquity of biotin-tagged molecules.