THE 3-DIMENSIONAL FOLDING OF THE TRANSFER RNA-LIKE STRUCTURE OF TOBACCO MOSAIC-VIRUS RNA - A NEW BUILDING PRINCIPLE APPLIED TWICE
THE 3-DIMENSIONAL FOLDING OF THE TRANSFER RNA-LIKE STRUCTURE OF TOBACCO MOSAIC-VIRUS RNA - A NEW BUILDING PRINCIPLE APPLIED TWICE
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DOI：
10.1002/j.1460-2075.1984.tb02182.x
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发表时间：
1984-01-01
期刊：
影响因子：
11.4
通讯作者：
BOSCH, L
中科院分区：
文献类型：
--
作者：
RIETVELD, K;LINSCHOOTEN, K;BOSCH, L
研究了烟草花叶病毒 (TMV) RNA 的 tRNA 样 3'' 末端的结构。通过化学修饰和酶消化来探测处理 tRNA 样特性的 3'' 末端片段。提出了最后 105 个核苷酸的二级结构模型。其他烟草花叶病毒RNA的相应区域可以折叠成相同的二级结构。给出了 tRNA 样结构的 3 维模型，并将其与之前提出的萝卜黄花叶病毒 (TYMV) RNA 和雀麦花叶 (BMV) RNA 的 tRNA 样 3'' 末端模型进行了比较。先前通过研究后一种 RNA 发现的新构建原理似乎在 TMV RNA 的 tRNA 样结构中应用了两次。识别 CTP、ATP:tRNA 核苷酸转移酶所需的最小长度的确定揭示了.apprx的大小。 100 个核苷酸与所提出的模型一致。
The structure of the tRNA-like 3'' terminus of tobacco mosaic virus (TMV) RNA was studied. A 3''-terminal fragment processing the tRNA-like properties was probed with chemical modification and enzymatic digestions. A model of the secondary structure is proposed for the last 105 nucleotides. The corresponding region of other tobamoviral RNA can be folded in an identical secondary structure. A 3-dimensional model for the tRNA-like structure is given which is compared with those proposed earlier for the tRNA-like 3'' termini of turnip yellow mosaic virus (TYMV) RNA and brome mosaic (BMV) RNA. A new building principle which was discovered previously by studying the latter RNA appears to be applied twice in the tRNA-like structure of TMV RNA. The determination of the minimal length requirement for recognition of CTP, ATP:tRNA nucleotidyltransferase reveals a size of .apprx. 100 nucleotides in agreement with the models proposed.