HOST PROTEIN-REQUIREMENTS FOR INVITRO SITE-SPECIFIC DNA INVERSION
HOST PROTEIN-REQUIREMENTS FOR INVITRO SITE-SPECIFIC DNA INVERSION
复制标题
DOI：
10.1016/0092-8674(86)90878-0
复制
发表时间：
1986-08-15
期刊：
影响因子：
64.5
通讯作者：
SIMON, MI
中科院分区：
文献类型：
--
作者：
JOHNSON, RC;BRUIST, MF;SIMON, MI
[沙门氏菌]鞭毛相变异是由发生在特定位点的重组事件介导的，导致DNA染色体片段的倒位。DNA底物分子上60 bp重组增强子序列的存在导致初始倒位速率的150倍刺激。转化所需的蛋白质组分已被纯化。它们包括21，000道尔顿重组酶（Hin）、12，000道尔顿宿主蛋白（因子II）和大肠杆菌HU的主要组蛋白样蛋白之一。重组的初始速率对HU的依赖性随重组增强子的位置而变化。HU，因子II，和增强子在促进位点特异性重组的作用进行了讨论。
Flagellar phase variation [in Salmonella] is mediated by a recombination event that occurs at specific sites leading to inversion of a chromosomal segment of DNA. The presence of a 60 bp recombinational enhancer sequence on the DNA substrate molecule results in a 150-fold stimulation in the initial rate of inversion. The protein components required for inversion have been purified. They include the 21,000 dalton recombinase (Hin), a 12,000 dalton host protein (Factor II), and one of the major histone-like proteins of Escherichia coli HU. The dependence of the initial rate of recombination on HU varies with respect to the location of the recombinational enhancer. The role of HU, Factor II, and the enhancer in facilitating site-specific recombination is discussed.