Hepatitis C virus genotypes and viremia and hepatocellular carcinoma in the United States.
Hepatitis C virus genotypes and viremia and hepatocellular carcinoma in the United States.
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美国的丙型肝炎病毒基因型以及病毒血症和肝细胞癌。
DOI：
10.1111/j.1572-0241.1999.01153.x
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Liang,TJ
中科院分区：
文献类型：
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作者：
Reid,AE;Koziel,MJ;Aiza,I;Jeffers,L;Reddy,R;Schiff,E;Lau,JY;Dienstag,JL;Liang,TJ
目的：丙型肝炎病毒（HCV）是公认的肝细胞癌（HCC）病因。 HCV 基因型在肝癌发生中的致病意义尚不清楚。本研究的目的是调查伴或不伴 HCC 的 HCV 相关肝硬化患者中 HCV 的基因型分布和病毒血症水平。 方法：总共研究了 28 例 HCV 感染的 HCC（HCC+）患者和 38 例 HCV 相关肝硬化不伴 HCC（HCC−）患者。 HCV基因型通过Okamoto基因型特异性聚合酶链式反应(PCR)方法和5'非翻译区(5'UTR)的限制性片段长度多态性(RFLP)进行评估。通过支链 DNA (bDNA) 测定对丙型肝炎病毒血症进行定量。 结果：使用冈本法，我们发现 64% 的 HCC+ 组和 74% 的 HCC− 组中存在基因型 1b，36% 的 HCC+ 组和 16% 的 HCC− 组同时感染了基因型 1b 和另一种基因型的组合。使用 RFLP 方法，我们在 41% 的 HCC+ 组和 24% 的 HCC− 组中发现了基因型 1b。其他基因型占HCC+组的18%，HCC−组的55%；没有鉴定出组合基因型。两种基因分型方法之间的一致性较差。两组之间的平均 bDNA 水平没有显着差异。结论：我们的研究表明，没有特定的 HCV 基因型与 HCC 相关，并且基因型似乎不会影响 HCV 相关 HCC 的发展。
OBJECTIVE:Hepatitis C virus (HCV) is a well recognized cause of hepatocellular carcinoma (HCC). The pathogenic significance of HCV genotypes in hepatocarcinogenesis is undefined. The aim of this study was to investigate the genotypic distribution and viremic level of HCV in patients with HCV-associated cirrhosis with or without HCC.METHODS:A total of 28 HCV-infected patients with HCC (HCC+) and 38 patients with HCV-associated cirrhosis without HCC (HCC−) were studied. HCV genotype was assessed by the genotype-specific polymerase chain reaction (PCR) method of Okamoto and restriction fragment length polymorphism (RFLP) of the 5′ untranslated region (5′ UTR). Hepatitis C viremia was quantitated with the branched-chain DNA (bDNA) assay.RESULTS:Using the Okamoto method, we found genotype 1b in 64% of the HCC+ group and 74% of the HCC− group, 36% of the HCC+ group and 16% of the HCC− group were coinfected with a combination of genotype 1b and another genotype. Using the RFLP method, we found genotype 1b in 41% of the HCC+ group and in 24% of the HCC− group. Other genotypes accounted for 18% of the HCC+ group and 55% of the HCC− group; no combination genotypes were identified. Poor concordance occurred between the two genotyping methods. Mean bDNA levels were not significantly different between the two groups.CONCLUSIONS:Our study demonstrates that no particular HCV genotypes were associated with HCC and genotype did not appear to influence the development of HCV-associated HCC.