Two immunoreactive binding proteins for insulin-like growth factors in human amniotic fluid: relationship to fetal maturity.
Two immunoreactive binding proteins for insulin-like growth factors in human amniotic fluid: relationship to fetal maturity.
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人羊水中胰岛素样生长因子的两种免疫反应性结合蛋白：与胎儿成熟度的关系。
DOI：
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发表时间：
1987
影响因子：
5.8
通讯作者：
M. H. Wood
中科院分区：
文献类型：
--
作者：
R. Baxter;Janet L. Martin;M. H. Wood
用IGF-Ⅰ亲和层析和高效反相层析从人羊水中分离纯化了一种胰岛素样生长因子（IGFs）结合蛋白。这种蛋白质的表观分子量为28 K非还原型和34 K还原型，与先前从羊水中纯化的结合蛋白和胎盘蛋白12具有相同的氨基末端。纯化的制剂（BP-28）以高亲和力结合两种IGF [Ka，IGF-I为6.55 +/- 2.24（+/- SD）L/nmol，IGF-II为3.23 +/- 1.05 L/nmol]，每mol BP-28中任一配体的结合位点约为0.5 mol。从人血浆中纯化的53 K IGF结合蛋白（BP-53）在RIA中与BP-28没有交叉反应，BP-28在RIA中与BP-53的摩尔交叉反应性小于0.1%。人羊水在两种测定中反应强烈。羊水样品的反相色谱分离表明，BP-28和BP-53免疫反应存在于单独的蛋白质。在40份选择涵盖广泛卵磷脂与鞘磷脂比值的妊娠晚期羊水样本中，BP-28和BP-53的平均浓度分别为37.6 +/- 17.6（+/- SD）和4.6 +/- 1.6 mg/L。BP-28和BP-53的水平与卵磷脂与鞘磷脂的比例之间存在显著的负相关性，表明这两种蛋白质的水平与胎儿成熟度之间存在关联。BP-28和BP-53水平之间也存在显著正相关。我们的结论是，28 K IGF结合蛋白从羊水中，像以前纯化的53 K结合蛋白，具有高亲和力的IGF-I和IGF-II，它共存于羊水中与BP-53或相关蛋白，这两种蛋白质的水平下降，随着胎儿成熟度的增加。
A binding protein for insulin-like growth factors (IGFs) has been purified from human amniotic fluid by IGF-I affinity chromatography and high performance reverse phase chromatography. This protein, with an apparent molecular mass of 28K nonreduced and 34K reduced, had an identical amino-terminus to a previously purified binding protein from amniotic fluid and to placental protein 12. The purified preparation (BP-28) bound both IGFs with high affinity [Ka, 6.55 +/- 2.24 (+/- SD) L/nmol for IGF-I and 3.23 +/- 1.05 L/nmol for IGF-II], with approximately 0.5 mol binding sites/mol BP-28 for either ligand. A 53K IGF-binding protein purified from human plasma (BP-53) did not cross-react in a RIA for BP-28, and BP-28 had less than 0.1% molar cross-reactivity in a RIA for BP-53. Human amniotic fluid reacted strongly in both assays. Fractionation of amniotic fluid samples by reverse phase chromatography showed that BP-28 and BP-53 immunoreactivities were present on separate proteins. In 40 third trimester amniotic fluid samples selected to cover a wide range of lecithin to sphingomyelin ratios, the mean concentrations of BP-28 and BP-53 were 37.6 +/- 17.6 (+/- SD) and 4.6 +/- 1.6 mg/L, respectively. Significant negative correlations were found between the levels of both BP-28 and BP-53 and the lecithin to sphingomyelin ratio, suggesting an association between the levels of both proteins and the degree of fetal maturity. A significant positive association was also found between the levels of BP-28 and BP-53. We conclude that the 28K IGF-binding protein from amniotic fluid, like the previously purified 53K binding protein, has high affinity for both IGF-I and IGF-II, that it coexists in amniotic fluid with BP-53 or a related protein, and that the levels of both proteins decline with increasing fetal maturity.
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DOI：
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发表时间：
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期刊：
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影响因子：
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DOI：
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影响因子：
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DOI：
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DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Mottola,C;MacDonald,RG;Brackett,JL;Mole,JE;Anderson,JK;Czech,MP
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