Bio-organic soil amendment promotes the suppression of Ralstonia solanacearum by inducing changes in the functionality and composition of rhizosphere bacterial communities
Bio-organic soil amendment promotes the suppression of Ralstonia solanacearum by inducing changes in the functionality and composition of rhizosphere bacterial communities
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DOI：
10.1111/nph.18221
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发表时间：
2022-06-08
期刊：
影响因子：
9.4
通讯作者：
Salles, Joana Falcao
中科院分区：
文献类型：
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作者：
Deng, Xuhui;Zhang, Na;Salles, Joana Falcao
刺激土壤对某些病原体的抑制性的发展是减少农业中农药使用的可持续解决方案。然而，了解抑制性的动态和导致病原体控制的机制在很大程度上仍然难以捉摸。在这里，我们研究了根际微生物组诱导细菌性枯萎病抑制的机制，在长期的田间试验中，连续施用生物有机肥（BFs）与施用化肥相比，引发了疾病抑制。我们进一步证明，在温室实验中，根际细菌群落的抑制性主要是由社区组成的变化，而不仅仅是由引入的生防菌株的丰度。宏基因组学方法显示，具有产生次生代谢产物能力的鞘氨醇单胞菌科和黄单胞菌科的成员在BF植物根际中富集，但仅在病原体入侵时富集。我们通过将属于鞘氨醇单胞菌科和黄单胞菌科的细菌分离物接种到有益土壤中，从而导致病原体丰度显著降低和非核糖体肽合成酶基因丰度增加，实验验证了这一观察结果。我们的结论是，启动的土壤微生物与BF修正培养反应细菌群落在番茄植物根际生物干扰。
Stimulating the development of soil suppressiveness against certain pathogens represents a sustainable solution toward reducing pesticide use in agriculture. However, understanding the dynamics of suppressiveness and the mechanisms leading to pathogen control remain largely elusive. Here, we investigated the mechanisms used by the rhizosphere microbiome induces bacterial wilt disease suppression in a long-term field experiment where continuous application of bio-organic fertilizers (BFs) triggered disease suppressiveness when compared to chemical fertilizer application. We further demonstrated in a glasshouse experiment that the suppressiveness of the rhizosphere bacterial communities was triggered mainly by changes in community composition rather than only by the abundance of the introduced biocontrol strain. Metagenomics approaches revealed that members of the families Sphingomonadaceae and Xanthomonadaceae with the ability to produce secondary metabolites were enriched in the BF plant rhizosphere but only upon pathogen invasion. We experimentally validated this observation by inoculating bacterial isolates belonging to the families Sphingomonadaceae and Xanthomonadaceae into conducive soil, which led to a significant reduction in pathogen abundance and increase in nonribosomal peptide synthetase gene abundance. We conclude that priming of the soil microbiome with BF amendment fostered reactive bacterial communities in the rhizosphere of tomato plants in response to biotic disturbance.