Murine germ cells do not require functional androgen receptors to complete spermatogenesis following spermatogonial stem cell transplantation.
Murine germ cells do not require functional androgen receptors to complete spermatogenesis following spermatogonial stem cell transplantation.
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DOI：
10.1210/endo.142.6.8317
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发表时间：
2001-06
期刊：
影响因子：
4.8
通讯作者：
Daniel S. Johnston;Lonnie D. Russell;P. Friel;Michael D. Griswold
中科院分区：
文献类型：
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作者：
Daniel S. Johnston;Lonnie D. Russell;P. Friel;Michael D. Griswold
精原干细胞移植技术被用来确定小鼠生殖细胞是否需要功能性雄激素受体来完成定性的正常精子发生。将睾丸女性化小鼠的睾丸细胞注射到表达功能性雄激素受体的无精子症小鼠生精小管中。移植后110天至200天对受者睾丸进行分析。在每个受体睾丸的生精小管中可以看到多个完整的、定性正常的供者来源的生精发生群体，这表明小鼠生殖细胞不需要功能正常的雄激素受体来完成生精。
The spermatogonial stem cell transplantation technique was employed to determine if murine germ cells require functional androgen receptors to complete qualitatively normal spermatogenesis. Testicular cells from testicular feminized mice were injected into the seminiferous tubules of azoospermic mice expressing functional androgen receptors. Recipient testes were analyzed between 110 and 200 days following transplantation. Multiple colonies of complete and qualitatively normal donor-derived spermatogenesis were seen within the seminiferous tubules of each recipient testis, demonstrating that murine germ cells do not require functional androgen receptors to complete spermatogenesis.