Polyethyleneimine-capped silver nanoclusters for microRNA oligonucleotide delivery and bacterial inhibition.
Polyethyleneimine-capped silver nanoclusters for microRNA oligonucleotide delivery and bacterial inhibition.
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用于 microRNA 寡核苷酸递送和细菌抑制的聚乙烯亚胺封端银纳米簇
DOI：
10.2147/ijn.s146968
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发表时间：
2017
影响因子：
8
通讯作者：
Chen Y
中科院分区：
文献类型：
--
作者：
Du C;Yan H;Liang J;Luo A;Wang L;Zhu J;Xiong H;Chen Y
高效、安全的非病毒基因传递系统是基因治疗应用于临床的前提。本文制备了聚乙烯亚胺包覆的银纳米团簇（PEI-AgNCs），并将其用于microRNA（miRNA）的递送。通过光致发光测定和透射电子显微镜对所得PEI-AgNC进行表征。细胞毒性测定显示PEI-AgNC表现出相对低的细胞毒性。有趣的是，PEI-AgNC被证实在HepG 2和293 A细胞中比PEI更有效地抑制miRNA模拟物。在这方面，通过使用PEI-AgNC将hsa-miR-21或hsa-miR-221模拟物（miR-21/221 m）转运到HepG 2细胞中。转染后通过定量实时聚合酶链反应测定miR-21/221的表达。与阴性对照组相比，PEI-AgNC/miR-21/221 m组miR-21/221水平更高。此外，AgNCs赋予PEI更强的抗菌活性，这一优势为PEI-AgNCs提供了在转染过程中防止细菌污染的潜力。此外，我们表明PEI-AgNC是可行的纳米材料，通过激光扫描共聚焦显微镜对细胞进行平面成像，表明作为理想的荧光探针跟踪转染行为的巨大潜力。这些结果表明，PEI-AgNC是有前途的和新的非病毒载体的基因传递。
Efficient and safe nonviral gene delivery systems are a prerequisite for the clinical application of therapeutic genes. In this paper, polyethyleneimine-capped silver nanoclusters (PEI-AgNCs) were prepared for the purpose of microRNA (miRNA) delivery. The resultant PEI-AgNCs were characterized by a photoluminescence assay and transmission electron microscopy. A cytotoxicity assay showed that PEI-AgNCs exhibit relatively low cytotoxicity. Interestingly, PEI-AgNCs were confirmed to transfect miRNA mimics more effectively than PEI in HepG2 and 293A cells. In this regard, hsa-miR-21 or hsa-miR-221 mimics (miR-21/221m) were transported into HepG2 cells by using PEI-AgNCs. The miR-21/221 expression was determined post-transfection by quantitative real-time polymerase chain reaction. Compared with the negative control, PEI-AgNCs/miR-21/221m groups exhibited higher miR-21/221 levels. In addition, AgNCs endow PEI with stronger antibacterial activity, and this advantage provided PEI-AgNCs the potential to prevent bacterial contamination during the transfection process. Furthermore, we showed that PEI-AgNCs are viable nanomaterials for plain imaging of the cells by laser scanning confocal microscopy, indicating great potential as an ideal fluorescent probe to track the transfection behavior. These results demonstrated that PEI-AgNCs are promising and novel nonviral vectors for gene delivery.