NONIDENTICAL DISTRIBUTION OF TRANSFERRIN AND FERRIC IRON IN HUMAN-BRAIN
NONIDENTICAL DISTRIBUTION OF TRANSFERRIN AND FERRIC IRON IN HUMAN-BRAIN
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DOI：
10.1016/0306-4522(88)90242-4
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发表时间：
1988-10-01
期刊：
影响因子：
3.3
通讯作者：
HERBERT, J
中科院分区：
文献类型：
--
作者：
DWORK, AJ;SCHON, EA;HERBERT, J
使用抗生物素蛋白-生物素免疫过氧化物酶技术和二氨基联苯胺强化普鲁士蓝法，我们比较了分布的转铁蛋白的三价铁在5个正常尸检的成年男性的大脑。所观察到的转铁蛋白的分布比：（1）组织化学可证实的三价铁;（2）报告的胎儿和新生儿人脑中的转铁蛋白;和（3）报告的转铁蛋白其他物种。转铁蛋白免疫反应存在于神经元，少突胶质细胞，星形胶质细胞，室管膜细胞，脉络丛上皮细胞，虽然不是在所有细胞的任何类型。铁，另一方面，是显而易见的，只有在少突胶质细胞，髓鞘，并可能在轴突。虽然转铁蛋白和铁染色相对较高的基底神经节和黑质，染色模式不同，纹状体传出纤维染色更重比neurologic的铁和较少的重比neurologic的转铁蛋白。大鼠的脉络丛显示出合成转铁蛋白，转铁蛋白染色很重，而铁则完全不染色。脉络丛中低铁和高转铁蛋白的发现表明，丛可能分泌转铁蛋白进入脑脊液，从而促进铁在轴突内的移位。此外，三价铁和转铁蛋白的不相同的分布表明，在人脑中，转铁蛋白除了将铁从细胞外液运输到细胞质外，还可能具有其他功能。
Using the avidin-biotin immunoperoxidase technique and a diaminobenzidine intensification of the Prussian Blue method, we have compared the distribution of transferrin to that of ferric iron in five normal autopsy brains from adult human males. The observed distribution of transferrin was considerably more widespread than: (1) that of histochemically demonstrable ferric iron; (2)that reported for transferrin in the fetal and neonatal human brain; and (3) that reported for transferrin other species. Transferrin immunoreactivity was present in neurons, oligodendrocytes, astrocytes, ependymal cells, and choroid plexus epithelial cells , although not in all cells of any type. Ferric iron, on the other hand, was demonstrable only in oligodendrocytes, in myelin sheaths, and possibly in axons. While staining for both transferrin and iron was relatively high in the basal ganglia nad substantia nigra, the pattern of staining differed, with striatal efferent fibers staining more heavily than the neuropil for iron and less heavily than the neuropil for transferrin. The choroid plexus, which in the rat has shown to synthesize transferrin, stained heavily for transferrin and not at all for iron. The findings of low iron and high transferrin in the choroid plexus suggest that the plexus may secrete transferrin into the cerebrospinal fluid, thereby facilitating the translocation of iron within the neuraxis. Furthermore, the nonidentical distribution of ferric iron and transferrin suggests that, in the human brain, transferrin may serve other functions besides the transport of iron from extracellular fluid to cytoplasm.