Cytotoxic effects of dental prosthesis grinding dust on RAW264.7 cells
Cytotoxic effects of dental prosthesis grinding dust on RAW264.7 cells
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DOI：
10.1038/s41598-020-71485-x
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发表时间：
2020-09
影响因子：
4.6
通讯作者：
Wei Wang;Tianshu Li;Xueqing Luo;Ke Zhang;Nanjue Cao;Keda Liu;Xiaoming Li;Yuhe Zhu
中科院分区：
文献类型：
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作者：
Wei Wang;Tianshu Li;Xueqing Luo;Ke Zhang;Nanjue Cao;Keda Liu;Xiaoming Li;Yuhe Zhu
呼吸道疾病，包括肺纤维化，矽肺和过敏性肺炎，可由长期接触假牙研磨粉尘引起。暴露于PMMA粉尘、Vitallium粉尘和牙本质瓷粉尘的毒性和致病性程度不同。对由这三种材料制成的牙科修复体的研磨粉尘的形态、粒度和元素组成进行了表征。评价了不同浓度的研磨粉尘（50、150、300、450和600 μg ml−l）对RAW 264.7巨噬细胞的不利影响，包括细胞形态和乳酸脱氢酶（LDH）和活性氧（ROS）产生的变化。这些材料制作的义齿磨削过程中释放的粉尘具有不同的形貌、粒径和元素组成。它们还在RAW 264.7巨噬细胞中诱导不同程度的细胞毒性。一种可能的细胞毒性机制是当灰尘颗粒穿透细胞时诱导脂质过氧化和质膜损伤。因此，经常使用这些材料的临床医生应穿戴适当的个人防护设备，以尽量减少暴露并降低这些颗粒物造成的健康风险。
Respiratory diseases, including pulmonary fibrosis, silicosis, and allergic pneumonia, can be caused by long-term exposure to dental prosthesis grinding dust. The extent of the toxicity and pathogenicity of exposure to PMMA dust, Vitallium dust, and dentin porcelain dust differs. The dust from grinding dental prosthesis made of these three materials was characterized in terms of morphology, particle size, and elemental composition. The adverse effects of different concentrations of grinding dust (50, 150, 300, 450, and 600 μg ml−l) on RAW264.7 macrophages were evaluated, including changes in cell morphology and the production of lactate dehydrogenase (LDH) and reactive oxygen species (ROS). The dust particles released by grinding dental prosthesis made of these materials had different morphologies, particle sizes, and elemental compositions. They also induced varying degrees of cytotoxicity in RAW264.7 macrophages. A possible cytotoxicity mechanism is the induction of lipid peroxidation and plasma membrane damage as the dust particles penetrate cells. Therefore, clinicians who regularly work with these materials should wear the appropriate personal protection equipment to minimize exposure and reduce the health risks caused by these particulates.