Pulmonary arterial dilation by inhaled NO: arterial diameter, NO concentration relationship.
Pulmonary arterial dilation by inhaled NO: arterial diameter, NO concentration relationship.
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吸入NO引起的肺动脉扩张：动脉直径、NO浓度关系。
DOI：
10.1152/jappl.2001.91.5.1948
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Dawson,CA
中科院分区：
文献类型：
--
作者：
Bentley,J;Rickaby,D;Haworth,ST;Hanger,CC;Dawson,CA
本研究的目的是确定一氧化氮（NO）浓度和血管直径对吸入一氧化氮引起的肺动脉扩张的依赖性。将分离的狗肺叶置于微焦x射线源和x射线探测器之间，并灌注血液或血浆。在对照条件下，在肺动脉灌注5 -羟色胺收缩和5 -羟色胺灌注同时吸入30 - 960份/百万一氧化氮时，向叶动脉注射不透射线造影剂。动脉直径测量是通过控制直径在300- 3400 μm范围内的血管的x射线图像获得的。血清素在研究的血管大小范围内收缩，直径平均减少约20%。吸入一氧化氮对血清素诱导的收缩的逆转作用与吸入一氧化氮浓度成正比，与血管大小成反比，在直径为3mm的血管中，对血浆的逆转作用大于对血液灌注的逆转作用。后者表明，在相当大的肺动脉中，血管内血红蛋白影响支气管肺泡-动脉腔内NO浓度梯度。这些数据提供了关于肺动脉平滑肌对肺内气体的可及性的信息，在未来的研究中，这些数据应该是建模肺内气体和肺动脉平滑肌细胞之间通信的有用数据库的一部分。
The objective of this study was to determine the nitric oxide (NO) concentration and vessel diameter dependence of the pulmonary arterial dilation induced by inhaled NO. Isolated dog lung lobes were situated between a microfocal X-ray source and X-ray detector and perfused with either blood or plasma. Boluses of radiopaque contrast medium were injected into the lobar artery under control conditions, when the pulmonary arteries were constricted by infusion of serotonin and when the serotonin infusion was accompanied by inhalation of from 30 to 960 parts/million NO. Arterial diameter measurements were obtained from X-ray images of vessels having control diameters in the 300- to 3,400-μm range. Serotonin constricted the vessels throughout the size range studied, with an average decrease in diameter of ∼20%. The fractional reversal of the serotonin-induced constriction by inhaled NO was directly proportional to inhaled NO concentration, inversely proportional to vessel size, and greater with plasma than with blood perfusion in vessels as large as 3 mm in diameter. The latter indicates that intravascular hemoglobin affected the bronchoalveolar-to-arterial luminal NO concentration gradient in fairly large pulmonary arteries. The data provide information regarding pulmonary arterial smooth muscle accessibility to intrapulmonary gas that should be useful as part of the database for modeling the communication between intrapulmonary gas and pulmonary arterial smooth muscle cells in future studies.