Mosaic Open Reading Frames in the Arabidopsis thaliana Mitochondrial Genome
Mosaic Open Reading Frames in the Arabidopsis thaliana Mitochondrial Genome
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DOI：
10.1515/bchm.1997.378.8.859
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发表时间：
1997-08
期刊：
影响因子：
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通讯作者：
J. Marienfeld;M. Unseld;P. Brandt;A. Brennicke
中科院分区：
文献类型：
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作者：
J. Marienfeld;M. Unseld;P. Brandt;A. Brennicke
在拟南芥的线粒体基因组中，由一个或多个线粒体基因复制的片段重组产生了八个镶嵌开放阅读框。这些重复代表未经编辑的序列，表明它们源自基因组 DNA 而不是 RNA。五个嵌合阅读框包含原始多肽的 N 末端和 5' 上游区域的信息。这些观察结果表明，植物线粒体中新的开放阅读框的产生可以通过重复基因片段的偶然延伸而相当容易地发生。正常拟南芥线粒体基因组中存在如此多的嵌合开放阅读框表明，此类重组序列仅偶尔且偶然地干扰花粉成熟过程中可能所需的峰值线粒体性能，并且通常不会引起细胞质雄性不育表型。
In the mitochondrial genome of Arabidopsis thaliana eight mosaic open reading frames arose by recombination of fragments duplicated from one or more mitochondrial genes. These duplications represent unedited sequences, suggesting their derivation from genomic DNA rather than RNA. Five of the chimeric reading frames contain the information for the N-terminus of the original polypeptide and 5' upstream regions. These observations suggest that the generation of novel open reading frames in plant mitochondria can occur rather easily by chance extensions of duplicated gene fragments. The presence of so many mosaic open reading frames in the normal Arabidopsis thaliana mitochondrial genome suggests that such recombined sequences interfere only occasionally and fortuitously with the peak mitochondrial performance presumably required during pollen maturation, and usually do not cause a cytoplasmic male sterile phenotype.