Norovirus GII-4 2006b Variant Circulating in Patients With Acute Gastroenteritis in Thailand During a 2006-2007 Study
Norovirus GII-4 2006b Variant Circulating in Patients With Acute Gastroenteritis in Thailand During a 2006-2007 Study
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DOI：
10.1002/jmv.21746
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发表时间：
2010-05-01
影响因子：
12.7
通讯作者：
Ushijima, Hiroshi
中科院分区：
文献类型：
--
作者：
Kittigul, Leera;Pombubpa, Kannika;Ushijima, Hiroshi
诺如病毒（NoV）被认为是儿童和成人急性胃肠炎的重要原因。 2006 年 1 月至 2007 年 2 月，泰国一家医院进行了一项为期 14 个月的研究，以确定所有年龄段的急性胃肠炎患者中 NoV 的患病率和遗传特征。基于逆转录巢式聚合酶链反应（RT-nested PCR），在收集的 273 份粪便样本中的 122 份（44.7%）中检测到了 NoV。 122例NoV阳性样本中，28例（23%）属于GI，79例（64.8%）属于GII，15例（12.2%）为GI和GII毒株混合感染。通过 RT 巢式 PCR 产物的 DNA 测序和系统发育分析，成功鉴定了 3 个 NoV GI 阳性和 42 个 NoV GII 阳性样本。对于 NoV GI，确定了两种基因型：GI-2（一个样本）和 GI-6（两个样本）。 NoV GII 可以进一步分为五种不同的基因型：GII-2（1 个样本）、GII-3（3 个样本）、GII-4（14 个样本）、GII-6（3 个样本）和 GII-17（2 个样本），以及一种未分类的基因型（19 个样本）。所有 NoV GII-4 菌株均与全球报道的 NoV GII-4 2006b 变体具有 88-98% 的核苷酸同一性。在已鉴定的 NoV 基因型中，一份共同感染的粪便样本显示出 NoV GI-6 和 GII-4 2006b。这项研究表明，NoV 作为急性胃肠炎患者的病原体具有重要作用。 NoV 感染的主要循环基因型是 GII-4 2006b 变种。 J. Med。病毒。 82:854 860, 2010。(C) 2010 Wiley-Liss, Inc.
Noroviruses (NoVs) are recognized as a significant cause of acute gastroenteritis in children and adults. A 14-month study, from January 2006 to February 2007, was undertaken in a hospital in Thailand to determine the prevalence and genetic characterization of NoVs in patients of all ages with acute gastroenteritis. Based on reverse transcription-nested polymerase chain reaction (RT-nested PCR), NoVs were detected in 122 of 273 (44.7%) collected stool samples. Of the 122 NoV-positive samples, 28 (23%) belonged to GI, 79 (64.8%) belonged to GII, and 15 (12.2%) were mixed infections of GI and GII strains. Three NoV GI-positive and 42 NoV GII-positive samples were characterized successfully by DNA sequencing of the RT-nested PCR products and phylogenetic analysis. For NoV GI, two genotypes were identified: GI-2 (one sample) and GI-6 (two samples). NoV GII could be classified further into five distinct genotypes: GII-2 (1 sample), GII-3 (3 samples), GII-4 (14 samples), GII-6 (3 samples), and GII-17 (2 samples), and one unclassified genotype (19 samples). All NoV GII-4 strains showed 88-98% nucleotide identity with NoV GII-4 2006b variants reported worldwide. Among genotypes of NoV characterized, one co-infected stool sample exhibited NoVs GI-6 and GII-4 2006b. This study suggests that there is an important role of NoVs as etiologic agents in patients with acute gastroenteritis. The predominant circulating genotype of NoV infections is GII-4 2006b variant. J. Med. Virol. 82:854 860, 2010. (C) 2010 Wiley-Liss, Inc.