Cloning and characterization of the majority of repetitive DNA in cotton (Gossypium L.).
Cloning and characterization of the majority of repetitive DNA in cotton (Gossypium L.).
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DOI：
10.1139/g95-156
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发表时间：
1995-12
期刊：
影响因子：
3.1
通讯作者：
X. Zhao;R. Wing;A. Paterson
中科院分区：
文献类型：
--
作者：
X. Zhao;R. Wing;A. Paterson
四倍体棉花（Gossypium barbadense L.）并且包含四倍体棉花基因组的30-36%的四倍体棉花基因组，是从用四种平端切割限制性内切酶的混合物消化的DNA的基因组文库中分离的。通过杂交和Southern杂交分析，将313个含有核重复序列的克隆分为1103个家族。这103个家庭的特点是基因组组织，甲基化模式，丰度和DNA变异。与许多其他真核生物基因组一样，散布的重复元件是棉花基因组中最丰富的重复DNA。与其他高等植物基因组相比，棉花基因组中缺乏高拷贝数（>10（4））的串联重复序列家族可能是其独特的特征。在棉花基因组中，散布重复序列倾向于甲基化，而串联重复序列似乎在很大程度上未甲基化。不同四倍体棉花物种之间重复DNA元件的库和总拷贝数的最小变化与四倍体棉花起源相对较近的假设一致。
Repetitive DNA elements representing 60-70% of the total repetitive DNA in tetraploid cotton (Gossypium barbadense L.) and comprising 30-36% of the tetraploid cotton genome were isolated from a genomic library of DNA digested with a mixture of four blunt-end cutting restriction enzymes. A total of 313 clones putatively containing nuclear repetitive sequences were classified into 1103 families, based on cross hybridization and Southern blot analysis. The 103 families were characterized in terms of genome organization, methylation pattern, abundance, and DNA variation. As in many other eukaryotic genomes, interspersed repetitive elements are the most abundant class of repetitive DNA in the cotton genome. Paucity of tandem repeat families with high copy numbers (>10(4)) may be a unique feature of the cotton genome as compared with other higher plant genomes. Interspersed repeats tend to be methylated, while tandem repeats seem to be largely unmethylated in the cotton genome. Minimal variation in repertoire and overall copy number of repetitive DNA elements among different tetraploid cotton species is consistent with the hypothesis of a relatively recent origin of tetraploid cottons.