Tubulin assembly induced by cobalt and zinc.
Tubulin assembly induced by cobalt and zinc.
复制标题
钴和锌诱导的微管蛋白组装。
DOI：
10.1016/s0006-291x(80)80095-7
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发表时间：
1980
影响因子：
3.1
通讯作者：
Himes,RH
中科院分区：
文献类型：
--
作者：
Haskins,KM;Zombola,RR;Boling,JM;Lee,YC;Himes,RH
在没有有机溶剂的情况下，在Mg（II）无效的条件下，Co（II）和Zn（II）都能刺激牛脑6S微管蛋白的组装。组装反应的产物取决于阳离子/微管蛋白的比例。在较低的比例下形成微管，但随着阳离子浓度的增加，主要产物是宽片的原丝。高离子强度、GDP、秋水仙碱和Ca（II）抑制了Co（II）中的组装反应。不像其他抑制剂会导致Co（II）诱导的微管解聚，Ca（II）加入到Co（II）诱导的微管中会形成宽片的原丝。
Both Co(II) and Zn(II) stimulate the assembly of bovine brain 6S tubulin in the absence of organic solvents, under conditions where Mg(II) is ineffective. The products of the assembly reaction depend on the cation/tubulin ratio. At lower ratios microtubules are formed, but as the cation concentration is increased, broad sheets of protofilaments are the predominate products. The assembly reaction in Co(II) is inhibited by high ionic strength, GDP, colchicine, and Ca(II). Unlike other inhibitors, which will cause depolymerization of Co(II)-induced microtubules, Ca(II) added to Co(II)-induced microtubules causes the formation of broad sheets of protofilaments.