M2 Macrophages Promote PDGFRβ(+) Pericytes Migration After Spinal Cord Injury in Mice via PDGFB/PDGFRβ Pathway.
M2 Macrophages Promote PDGFRβ(+) Pericytes Migration After Spinal Cord Injury in Mice via PDGFB/PDGFRβ Pathway.
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M2 巨噬细胞通过 PDGFB/PDGFR beta 途径促进小鼠脊髓损伤后 PDGFR beta( ) 周细胞迁移
DOI：
10.3389/fphar.2021.670813
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发表时间：
2021
影响因子：
5.6
通讯作者：
Jing J
中科院分区：
文献类型：
--
作者：
Li Z;Zheng M;Yu S;Yao F;Luo Y;Liu Y;Tian D;Cheng L;Jing J
血小板衍生生长因子受体β阳性(PDGFRβ+)周细胞形成纤维性瘢痕，阻碍脊髓损伤后轴突再生。然而，PDGFRβ+周细胞迁移到损伤核心的机制尚不清楚。在此，我们研究了巨噬细胞极化对脊髓损伤后PDGFRβ+周细胞迁移的影响及其机制。伤后3、7、14d，巨噬细胞与损伤中心β+周细胞的时空分布密切相关。巨噬细胞在3dpi和7dpi出现M2极化，而在14dpi出现M1极化。脊髓损伤后和巨噬细胞M2极化后，血小板衍生生长因子B(PDGFB)的表达明显增加。外源性PDGFB和M2巨噬细胞条件培养液对PDGFFRβ+周细胞迁移的促进作用可被PDGFFRβ特异性抑制剂SU16f阻断。这些结果表明，M2巨噬细胞可以分泌作用于PDGFFRβ的PDGFB，促进PDGFFRβ+周细胞的迁移，该作用可被PDGFFRβ特异性抑制剂SU16f所阻断。PDGFB/PDGFFRβ通路是治疗脊髓损伤的新靶点。
Platelet derived growth factor receptor β positive (PDGFRβ+) pericytes form fibrotic scar, which prevents axonal regeneration after spinal cord injury (SCI). However, the mechanism by which PDGFRβ+ pericytes migrate to the injury core is unclear. Here, we investigated the effect and mechanism of macrophages polarization on PDGFRβ+ pericytes migration after SCI. Macrophages were closely related to the spatiotemporal distribution of PDGFRβ+ pericytes in the injury core at 3, 7, and 14 days postinjury (dpi). Macrophages appeared M2 polarization at 3 and 7 dpi while M1 polarization at 14 dpi. The expression of platelet derived growth factor B (PDGFB) was significantly increased after SCI and after macrophages M2 polarization. The promoting effect of exogenous PDGFB and M2 macrophages conditioned medium on PDGFRβ+ pericytes migration could be blocked by SU16f, a PDGFRβ specific inhibitor. These findings indicate that M2 macrophages can secrete PDGFB acting on PDGFRβ to promote PDGFRβ+ pericytes migration, which can be blocked by a PDGFRβ specific inhibitor SU16f. The PDGFB/PDGFRβ pathway is a promising new target for the treatment of SCI.
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影响因子：
4.6
作者：
Hiroi M;Sakaeda Y;Yamaguchi H;Ohmori Y
通讯作者：
Ohmori Y
影响因子：
64.5
作者：
Dias DO;Kim H;Holl D;Werne Solnestam B;Lundeberg J;Carlén M;Göritz C;Frisén J
通讯作者：
Frisén J
影响因子：
15.9
作者：
Abramsson, A;Lindblom, P;Betsholtz, C
通讯作者：
Betsholtz, C
影响因子：
5.6
作者：
Fan, Hua;Wu, Qiong;Wang, Youhua
通讯作者：
Wang, Youhua
神经系统中的PDGF/PDGFR轴。
影响因子：
10.6
作者：
Sil S;Periyasamy P;Thangaraj A;Chivero ET;Buch S
通讯作者：
Buch S