How to track spermatozoa using high-speed visual feedback
How to track spermatozoa using high-speed visual feedback
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DOI：
10.1109/iembs.2008.4649106
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发表时间：
2008-10
期刊：
影响因子：
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通讯作者：
H. Oku;M. Ishikawa;N. Ogawa;Kogiku Shiba;Manabu Yoshida
中科院分区：
文献类型：
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作者：
H. Oku;M. Ishikawa;N. Ogawa;Kogiku Shiba;Manabu Yoshida
在本文中，我们报道了如何在1 kHz的帧速率下使用高速视觉反馈来快速而有力地跟踪游泳的海鞘精子。海鞘类精子通过旋转鞭毛50次游到300μm/S/S。这种激烈的游泳方式阻碍了稳定的图像观察，并使我们以前开发的视觉跟踪系统难以可靠地跟踪它们。在这里，我们描述了我们如何克服这些问题，使用图像处理技术，使用高速视觉反馈，实现了对180多个S快速、小的腹水精子的稳定跟踪。
In this paper, we report how to track quickly and vigorously swimming ascidian spermatozoa using high-speed visual feedback at a frame rate of 1 kHz. Ascidian spermatozoa swim as fast as 300 μm/s by rotating their flagella 50 times/s. This vigorous swimming style has prevented stable image observation and made it difficult to track them reliably with our previously developed visual tracking system. Here, we describe how we overcame these problems using image processing techniques to achieve stable tracking of fast, small ascidian spermatozoa for more than 180 s using high-speed visual feedback.