Circus movement in dissociated embryonic cells of a teleost, Oryzias latipes.
Circus movement in dissociated embryonic cells of a teleost, Oryzias latipes.
复制标题
硬骨鱼 Oryzias latipes 的分离胚胎细胞中的马戏团运动。
作者：
N. Fujinami;T. Kageyama
淡水鱼 Oryzias latipes 的分离的早期胚胎细胞突出透明的叶状伪足，这些伪足倾向于在传播波中围绕细胞圆周旋转。来自晚期囊胚或原肠胚的细胞持续表现出这种“马戏团运动”，而大多数直到早期囊胚的细胞都是圆形的。叶状足的线速度是通过延时摄影显微摄影来估计的。随着细胞直径的增加，速度略有增加。还定量研究了浸入介质的 pH、温度和渗透压对运动的影响。当浸入 pH 值低于 5 的介质中时，细胞会变成圆形，并且不会形成叶状足泡。速度会随着温度的降低而降低，但即使在 5 摄氏度下，运动也会继续。置于高渗盐溶液中的细胞会变成锯齿形，不会持续表现出马戏团运动。
The dissociated early embryonic cells of the fresh water fish, Oryzias latipes, protrude hyaline lobopodia, which tend to rotate around the cell circumference in a propagating wave. Cells from late blastula or gastrula continuously show this "circus movement", while most cells up to early blastula are rounded. The linear velocity of the lobopodium was estimated by means of time-lapse cinemicrography. The velocity increases slightly as cell diameter increases. The effects of pH, temperature and osmotic pressure of the immersion media on the movement were also quantitatively investigated. Cells become rounded and do not form lobopodial blebs when immersed in media below pH 5. The velocity is reduced by decreasing temperature, but the movement continues even at 5 degrees C. Cells placed in hypertonic salt solutions become crenated and do not continuously demonstrate the circus movement.