Long noncoding RNA BX357664 regulates cell proliferation and epithelial-to-mesenchymal transition via inhibition of TGF-β1/p38/HSP27 signaling in renal cell carcinoma.
Long noncoding RNA BX357664 regulates cell proliferation and epithelial-to-mesenchymal transition via inhibition of TGF-β1/p38/HSP27 signaling in renal cell carcinoma.
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长非编码 RNA BX357664 通过抑制肾细胞癌中的 TGF-β 1/p38/HSP27 信号传导来调节细胞增殖和上皮间质转化
DOI：
10.18632/oncotarget.12937
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发表时间：
2016-12-06
期刊：
影响因子：
--
通讯作者：
Wang Z
中科院分区：
文献类型：
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作者：
Liu Y;Qian J;Li X;Chen W;Xu A;Zhao K;Hua Y;Huang Z;Zhang J;Liang C;Su S;Li P;Shao P;Li J;Qin C;Wang Z
反义长链非编码RNA（lncRNA）在多种恶性肿瘤的发生发展中起着重要的调控作用。然而，lncRNA在肾细胞癌（RCC）中的作用及其机制尚不清楚。本研究旨在检测lncRNA BX 357664在肾细胞癌中的表达，并探讨其在肾细胞癌细胞系中的功能。因此，根据先前的微阵列分析和定性实时聚合酶链反应，BX 357664在RCC中下调。在上调BX 357664后，检测到RCC细胞的迁移、侵袭和增殖能力降低。此外，进行Western blot分析以确定BX 357664对RCC中上皮-间充质转化、基质金属蛋白酶2、基质金属蛋白酶9和转化生长因子-β 1（TGF-β1）/p38/HSP 27信号通路的影响。随后，在存在BX 357664的情况下上调TGF-β1的蛋白水平可以挽救由BX 357664介导的恶性行为的抑制，表明BX 357664将其抑制作用归因于TGF-β1的抑制。因此，我们研究了一种新的lncRNA BX 357664，其可能通过阻断TGF-β1/p38/HSP 27通路而在RCC转移和进展中发挥抑制作用。
Antisense long noncoding RNAs (lncRNAs) are reported to play a regulating role in carcinogenesis of various human malignancies. However, the function of lncRNAs and their underlying mechanism in renal cell carcinoma (RCC) is still unknown. The aims of this study are to investigate the expression of lncRNA BX357664 in RCC and to explore its function in RCC cell lines. As a result, BX357664 was downregulated in RCC according to previous microarray analysis and qualitative real-time polymerase chain reaction. After the upregulation of BX357664, reduced migration, invasion, and proliferation capabilities in RCC cells were detected. Furthermore, Western blot analysis was conducted to identify the influence of BX357664 on epithelial-to-mesenchymal transition, matrix metalloproteinase 2, matrix metalloproteinase 9, and transforming growth factor-beta 1 (TGF-β1)/p38/HSP27 signaling pathway in RCC. Subsequently, upregulating the protein level of TGF-β1 in the presence of BX357664 could rescue the suppression of the malignant behavior mediated by BX357664, indicating that BX357664 attributed its inhibitory role to the suppression of TGF-β1. Therefore, we investigated a novel lncRNA BX357664, which might exhibit its inhibitory role in RCC metastasis and progression by blocking the TGF-β1/p38/HSP27 pathway.