Evolutionary rates and genetic diversities of mixed potyviruses in Narcissus
Evolutionary rates and genetic diversities of mixed potyviruses in Narcissus
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DOI：
10.1016/j.meegid.2016.08.036
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发表时间：
2016-11-01
影响因子：
3.2
通讯作者：
Tomimura, Kenta
中科院分区：
文献类型：
--
作者：
Ohshima, Kazusato;Nomiyama, Rei;Tomimura, Kenta
没有人试图评估从自然界中混合感染宿主收集的病毒的进化速度、时间尺度和多样性。水仙属植物是单子叶植物，对几种病毒敏感。本研究在日本采集了叶呈花叶状或条纹状的水仙（narcissus tazetta var. chinensis），利用水仙病毒特异性引物研究了水仙病毒的感染情况。水仙单株经常被不同病毒、不同分离株和同一病毒准种混合感染。病毒为水痘病毒科的水痘病毒和一种大病毒，即水痘晚黄病毒（NLSYV）、水痘黄条纹病毒（NYSV）、水痘变性病毒（NDV）、水痘a病毒（CyEVA）和水痘潜伏病毒（NLV）。不同病毒种外壳蛋白编码区遗传多样性不同；NYSV和CyEVA的多样性最多，NDV的多样性最少。5种水仙花病毒的进化速率为1.33 ~ 7.15 × 10(-3) nt/位点/年，进化速率相似。最近的共同祖先（TMRCAs）在不同的病毒种类之间存在差异；NYSV和CyEVA年龄最大，NDV年龄最小。因此，病毒的TMRCAs的年龄与核苷酸多样性的大小密切相关。(C) 2016 Elsevier b.v.版权所有
There is no attempt to evaluate evolutionary rates, timescales and diversities of viruses collected from mixedly infected hosts in nature. Plants of the genus Narcissus are a monocotyledon and are susceptible to several viruses. In this study, narcissus plants (Narcissus tazetta var. chinensis) showing mosaic or striping leaves were collected in Japan, and these were investigated for potyvirus infections using potyvirus-specific primers. Individual narcissus plants were found frequently to be mixedly infected with different potyviruses, different isolates and quasispecies of same virus. The viruses were potyviruses and a macluravirus in the family Potyviridae, namely Narcissus late season yellows virus (NLSYV), Narcissus yellow stripe virus (NYSV), Narcissus degeneration virus (NDV), Cyrtanthus elatus virus A (CyEVA) and Narcissus latent virus (NLV). Genetic diversities of coat protein coding region of different virus species were different; NYSV and CyEVA were most diverse whereas NDV was least. Evolutionary rates of all five narcissus viruses were 1.33-7.15 x 10(-3) nt/site/year andwere similar. The most recent common ancestors (TMRCAs) varied between virus species; NYSV and CyEVA were the oldest whereas NDV was the youngest. Thus, the oldness of TMRCAs of the viruses correlated well with the greatness of nucleotide diversities. (C) 2016 Elsevier B. V. All rights reserved.