AN ACTION SPECTRUM FOR CELL KILLING AND PYRIMIDINE DIMER FORMATION IN CHINESE HAMSTER V‐79 CELLS
AN ACTION SPECTRUM FOR CELL KILLING AND PYRIMIDINE DIMER FORMATION IN CHINESE HAMSTER V‐79 CELLS
复制标题
中国仓鼠 V-79 细胞杀伤和嘧啶二聚体形成的作用谱
DOI：
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发表时间：
1979
期刊：
影响因子：
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通讯作者：
R. B. Setlow
中科院分区：
文献类型：
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作者：
R. H. Rothman;R. B. Setlow
We have determined action spectra for pyrimidine dimer formation and loss of colony‐forming ability in Chinese Hamster V‐79 cells and have found a very strong correlation between the two. These data are consistent with the notion that damage to DNA is the principle cause of cell death and that the most important type of damage is the pyrimidine dimer. While the shape of the V‐79 spectra mimics that of action spectra for bacteria. phage, and purified DNA, V‐79 cells are about twice as sensitive to radiation at long wavelengths, relative to the sensitivity at 265 nm. However, if the action spectra are normalized to 297 nm. a wavelength included in the solar spectrum, the two sets of action spectra would coincide at wavelengths relevant to human skin‐cancer. Thus an action spectrum based on microorganisms should be adequate for extrapolation to humans in terms of risk due to ozone depleteion.
